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Protein profiling in a set of wild rice species and rice cultivars: a stepping stone to protein quality improvement
Cereal Research Communications ( IF 1.6 ) Pub Date : 2022-05-23 , DOI: 10.1007/s42976-022-00273-2
Rajvir Kaur , Rupinder Kaur , Neerja Sharma , Neelam Kumari , Renu Khanna , Gurjeet Singh

In this study, a total of 118 genotypes, including 90 wild accessions belonging to AA genome (O. glaberrima, O. barthii, O. nivara, O. rufipogon, O. longistaminata, O. meridionalis, O. glumaepatula) and CC genome (O. officinalis), 19 non-basmati genotypes and nine basmati cultivars were analyzed for their protein content and grain characteristics in brown rice. The protein content in wild rice accessions was appreciably high as 20.8% followed by non-basmati and (11.5%) and basmati (11%) cultivars. Thousand-grain weight and grain breadth (GB) were highest in wild rice accessions (30.4gm and 2.9 mm), while grain length and L: B were highest in basmati cultivars (8.9 mm and 6.2). Fractionation of storage proteins and SDS gel electrophoresis revealed that significant differences in the protein content between the wild rice species and cultivated rice genotypes were mainly due to differences in their glutelin contents and high molecular weight glutelin polypeptides. The presence of characteristic bands in selected accessions is a useful parameter for identification of rice germplasm. The wild species that had high protein content has potential to increase the nutritional quality of rice through interspecific crosses in future breeding programs.



中文翻译:

一组野生稻品种和水稻品种的蛋白质分析:蛋白质质量改进的垫脚石

在这项研究中,共有 118 个基因型,包括 90 个属于 AA 基因组(O. glaberrima、O. barthii、O. nivara、O. rufipogon、O. longistaminata、O. meridionalis、O. glumaepatula)和 CC 基因组的野生种质( O. officinalis),分析了 19 个非巴斯马蒂基因型和 9 个巴斯马蒂品种在糙米中的蛋白质含量和籽粒特征。野生稻种质中的蛋白质含量明显高达 20.8%,其次是非巴斯马蒂品种(11.5%)和巴斯马蒂品种(11%)。野生稻种质的千粒重和粒宽(GB)最高(30.4gm和2.9mm),而basmati品种的粒长和L:B最高(8.9mm和6.2)。贮藏蛋白分级分离和SDS凝胶电泳表明,野生稻品种和栽培稻基因型之间蛋白质含量的显着差异主要是由于它们的谷蛋白含量和高分子量谷蛋白多肽的差异。所选种质中特征条带的存在是鉴定水稻种质的有用参数。在未来的育种计划中,具有高蛋白质含量的野生物种有可能通过种间杂交来提高水稻的营养品质。

更新日期:2022-05-24
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