Collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), ultraviolet photodissociation (UVPD), electron capture dissociation and electron detachment dissociation (EDD) experiments were conducted on a set of phosphopeptides, in a Fourier transform ion cyclotron resonance mass spectrometer. The fragmentation patterns were compared and varied according to the fragmentation mechanisms and the composition of the peptides. CAD and IRMPD produced similar fragmentation profiles of the phosphopeptides, while UVPD produced a large number of complementary fragments. Electron-based dissociation techniques displayed lower fragmentation efficiencies, despite retaining the labile phosphate group, and drastically different fragmentation profiles. EDD produced complex spectra whose interpretation proved challenging.

中文翻译：

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用于分析磷酸肽的多模式串联质谱技术

在傅里叶变换离子回旋共振质谱仪中对一组磷酸肽进行碰撞激活解离 (CAD)、红外多光子解离 (IRMPD)、紫外光解离 (UVPD)、电子捕获解离和电子脱离解离 (EDD) 实验。根据片段化机制和肽的组成，比较和改变片段化模式。CAD 和 IRMPD 产生了相似的磷酸肽片段化谱，而 UVPD 产生了大量互补片段。尽管保留了不稳定的磷酸基团，但基于电子的解离技术显示出较低的碎裂效率，以及截然不同的碎裂谱。EDD 产生了复杂的光谱，其解释被证明具有挑战性。