Isoacteoside is a caffeoyl phenylethanoide glycoside found in various plant parts, such as the flower of Magnolia denudata. In particular, magnolia has been studied for its anti-obesity, anticancer, and anti-inflammatory effects. However, isoacteoside has not been extensively studied, except for its anti-inflammatory effects. In this study, the anti-obesity effects of isoacteoside were investigated in 3T3-L1 mouse pre-adipocytes. Isoacteoside treatment did not induce cytotoxicity in 3T3-L1 cells up to a concentration of 1000 μM. The anti-obesity effects on 3T3-L1 cells were confirmed using oil red O staining. In addition, the expression of obesity-related proteins and genes, such as peroxisome proliferator-activated acceptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and perilipin (PLIN1), was determined by western blotting and qRT-PCR assays to confirm the anti-obesity effects of isoacteoside. The results of this study suggest that isoacteoside, a natural substance isolated from plant extracts, is not highly toxic to normal cells, such as pre-adipocytes, and displays anti-obesity effects in vitro.

中文翻译：

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异乳糖苷对 3T3-L1 脂肪细胞的抗肥胖作用

Isoacteoside 是一种咖啡酰苯乙醇苷，存在于各种植物部位，例如 Magnolia denudata 的花。特别是，已经研究了木兰的抗肥胖、抗癌和抗炎作用。然而，除了其抗炎作用外，异阿糖苷还没有被广泛研究。在这项研究中，在 3T3-L1 小鼠前脂肪细胞中研究了异阿糖苷的抗肥胖作用。Isoacteoside 处理在 3T3-L1 细胞中不诱导细胞毒性，浓度高达 1000 μM。使用油红O染色证实了对3T3-L1细胞的抗肥胖作用。此外，肥胖相关蛋白和基因的表达，如过氧化物酶体增殖物激活受体 γ (PPARγ)、CCAAT/增强子结合蛋白 α (C/EBPα) 和 perilipin (PLIN1)，通过蛋白质印迹和 qRT-PCR 测定来确定异乳糖苷的抗肥胖作用。这项研究的结果表明，从植物提取物中分离出来的天然物质异醋酸苷对前脂肪细胞等正常细胞没有高毒性，并在体外显示出抗肥胖作用。