The main objective of this research was to conduct a dose–response modeling between the internal dose of measured blood Cd, As, Hg, Ni, and Cr and hormonal response of serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH). The study included 207 male participants from subjects of 5 different cohorts (patients with prostate, testicular, and pancreatic cancer, patients suffering from various thyroid and metabolic disorders, as well as healthy volunteers), enrolled from January 2019 to May 2021 at the Clinical Centre of Serbia in Belgrade, Serbia. Benchmark dose–response modeling analysis was performed with the PROAST software version 70.1, showing the hormone levels as quantal data. The averaging technique was applied to compute the Benchmark dose (BMD) interval (BMDI), with benchmark response set at 10%. Dose-response relationships between metal/metalloid blood concentration and serum hormone levels were confirmed for all the investigated metals/metalloid and hormones. The narrowest BMDI was found for Cd-testosterone and Hg-LH pairs, indicative of high confidence in these estimates. Although further research is needed, the observed findings demonstrate that the BMD approach may prove to be significant in the dose–response modeling of human data.

本研究的主要目的是在测量的血液 Cd、As、Hg、Ni 和 Cr 的内部剂量与血清睾酮、促黄体生成素 (LH) 和促卵泡激素的激素反应之间进行剂量反应模型。 FSH)。该研究包括来自 5 个不同队列（前列腺癌、睾丸癌和胰腺癌患者，患有各种甲状腺和代谢疾病的患者，以及健康志愿者）的 207 名男性参与者，他们于 2019 年 1 月至 2021 年 5 月在临床中心注册塞尔维亚贝尔格莱德，塞尔维亚。使用 PROAST 软件 70.1 版进行基准剂量反应模型分析，将激素水平显示为量子数据。应用平均技术来计算基准剂量 (BMD) 间隔 (BMDI)，基准响应设置为 10%。对于所有研究的金属/类金属和激素，金属/类金属血液浓度和血清激素水平之间的剂量反应关系得到了证实。发现 Cd-睾酮和 Hg-LH 对的 BMDI 最窄，表明对这些估计的置信度很高。尽管需要进一步研究，但观察到的结果表明，BMD 方法可能被证明在人体数据的剂量反应模型中具有重要意义。