Here we seek to gain insight into changes in the plasma membrane of live cells upon the application of osmotic stress using Laurdan, a fluorescent probe that reports on membrane organization, hydration, and dynamics. It is known that the application of osmotic stress to lipid vesicles causes a decrease in Laurdan’s generalized polarization (GP), which has been interpreted as an indication of membrane stretching. In cells, we see the opposite effects, as GP increases when the osmolarity of the solution is decreased. This increase in GP is associated with the presence of caveolae, which are known to disassemble and flatten in response to osmotic stress.

中文翻译：

---

通过劳丹一般偏振测量探测渗透应激对活细胞质膜的影响

在这里，我们试图使用 Laurdan（一种报告膜组织、水合和动力学的荧光探针）深入了解施加渗透压后活细胞质膜的变化。众所周知，对脂质囊泡施加渗透应激会导致劳丹广义极化 (GP) 降低，这被解释为膜拉伸的迹象。在细胞中，我们看到相反的效果，因为当溶液的渗透压降低时，GP 会增加。GP 的增加与小凹的存在有关，众所周知，小凹会因渗透压而分解并变平。