The study aimed to assess the effects of vitamin E (VE) supplementation and fat source on fatty acid (FA) composition, VE concentrations, and antioxidant capacity in plasma and tissues of pigs fed to a heavy slaughter weight (150 kg). A total of 64 pigs (32 barrows, 32 gilts; 28.41 ± 0.83 kg) were blocked by sex and weight, and randomly assigned to 1 of 8 dietary treatments (n = 8 per treatment) in a 4 × 2 factorial arrangement. Fat sources included corn-starch (CS), 5% tallow (TW), 5% distiller’s corn oil (DCO), and 5% coconut oil (CN); VE supplementation levels were 11 and 200 ppm. Five-phase diets were formulated to meet requirement estimates of NRC (2012) and fed to pigs for each period of 25 kg from 25 to 150 kg. Increasing VE supplementation level increased C16:1 (P < 0.05) content but decreased C20:0 (P < 0.05) content in backfat and belly fat while in liver it increased C17:0 (P < 0.05) but decreased C18:0 (P < 0.05). Compared to the pigs fed the CS diet, the pigs fed the CN diet had greater (P < 0.05) content of total saturated FA, the pigs fed the DCO diet had greater (P < 0.05) content of total polyunsaturated FA content and iodine value, and the pigs fed the TW diet had greater (P < 0.05) content of total monounsaturated FA in backfat, belly fat, and liver. Plasma VE concentrations increased linearly (P < 0.05) with increasing length of feeding but faster (P < 0.05) in the pigs fed the CN and TW diets compared to the CS and DCO diets within the 200 ppm VE level; the pigs fed the DCO diet had the highest plasma VE concentrations (P < 0.05) from Phase 2 to Phase 5 within the 11 ppm VE level. The VE concentrations in liver and loin muscle (P < 0.05) increased with increasing dietary VE level from 11 to 200 ppm but it was not affected by dietary fat source. There was no effect of VE supplementation and fat source on antioxidant capacity in plasma and liver except that pigs fed the DCO diet had greater liver SOD activity (P < 0.05) than the pigs fed the CN diet. In conclusion, dietary VE supplementation did not affect FA profile in backfat, belly fat and liver consistently while dietary FA composition with different fat sources affected much of the FA profile in backfat, belly fat, and liver. The higher level of VE supplementation increased liver and muscle VE concentrations and dietary fat sources affected plasma VE concentrations differently (P < 0.05) wherein the TW and CN diets increased the VE absorption greater than the DCO diet.

中文翻译：

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日粮维生素 E 和脂肪补充剂对 150 公斤重屠宰体重的生长肥育猪的影响：II。血浆和组织的组织脂肪酸谱、维生素 E 浓度和抗氧化能力

该研究旨在评估补充维生素 E (VE) 和脂肪源对大屠宰体重 (150 kg) 的猪血浆和组织中脂肪酸 (FA) 组成、VE 浓度和抗氧化能力的影响。共有 64 头猪（32 头手推车，32 头后备母猪；28.41 ± 0.83 kg）被性别和体重阻止，并以 4 × 2 因子排列随机分配到 8 个日粮处理中的 1 个（每个处理 n = 8）。脂肪来源包括玉米淀粉 (CS)、5% 牛油 (TW)、5% 玉米酒糟油 (DCO) 和 5% 椰子油 (CN)；VE 补充水平为 11 和 200 ppm。制定了五阶段日粮以满足 NRC（2012 年）的需求估计，并在 25 至 150 公斤的每个 25 公斤期间喂给猪。增加 VE 补充水平会增加 C16:1 (P < 0.05) 含量，但会降低 C20:0 (P < 0. 05) 背膘和腹部脂肪含量，肝脏中C17:0 含量增加（P < 0.05），而C18:0 含量降低（P < 0.05）。与饲喂 CS 日粮的猪相比，饲喂 CN 日粮的猪总饱和 FA 含量更高（P < 0.05），饲喂 DCO 日粮的猪总多不饱和 FA 含量更高（P < 0.05），碘值，而饲喂 TW 日粮的猪在背膘、腹部脂肪和肝脏中的总单不饱和 FA 含量较高（P < 0.05）。与饲喂 200 ppm VE 水平的 CS 和 DCO 日粮相比，饲喂 CN 和 TW 日粮的猪的血浆 VE 浓度随着饲喂时间的增加呈线性增加（P < 0.05），但速度更快（P < 0.05）；饲喂 DCO 饮食的猪在 11 ppm VE 水平内从第 2 阶段到第 5 阶段具有最高的血浆 VE 浓度 (P < 0.05)。肝脏和腰部肌肉中的 VE 浓度 (P < 0.05) 随着日粮 VE 水平从 11 到 200 ppm 的增加而增加，但不受日粮脂肪来源的影响。除了饲喂 DCO 日粮的猪比饲喂 CN 日粮的猪具有更高的肝脏 SOD 活性（P < 0.05）外，补充 VE 和脂肪源对血浆和肝脏的抗氧化能力没有影响。总之，膳食 VE 补充剂不会始终影响背膘、腹部脂肪和肝脏中的 FA 谱，而具有不同脂肪来源的膳食 FA 成分会影响背膘、腹部脂肪和肝脏中的大部分 FA 谱。较高水平的 VE 补充剂增加了肝脏和肌肉 VE 浓度，而膳食脂肪来源对血浆 VE 浓度的影响不同（P < 0.