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Antibiofilm and cytotoxic potential of extracellular biosynthesized gold nanoparticles using actinobacteria Amycolatopsis sp. KMN
Preparative Biochemistry & Biotechnology ( IF 2.9 ) Pub Date : 2022-05-20 , DOI: 10.1080/10826068.2022.2076244
Faezeh Kabiri, Seyyed Soheil Aghaei, Ahmad Ali Pourbabaee, Mohammad Soleimani, Tahereh Komeili Movahhed

Abstract

This study intends to biosynthesize gold nanoparticles (AuNPs) using Amycolatopsis sp. KMN and to investigate its potential antibiofilm, cytotoxic and antioxidant activities. The physicochemical characterization of biosynthesize AuNPs was identified by UV-Visible, energy-dispersive X-ray, and Fourier transform infrared spectroscopy, as well as high-resolution transmission electron microscopy, X-ray diffraction, zeta potential, and dynamic light scattering methods. Crystal violet assay and scanning electron microscopy showed that the AuNPs with a particle size of 44.4 nm have a strong antibiofilm activity (at 750 µg/ml concentration) against bacteria strains viz Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The result also demonstrated strong cytotoxic activity against two cell lines, MCF-7 and HT-29. The MTT test result displayed that over a period of 48 hr, the IC50 of AuNPs was 600 and 300 µg/ml for MCF-7 and HT-29 cell lines, respectively. The IC50 of AuNPs against DPPH was 46.87 µg/ml. This is the first report that examines Amycolatopsis sp. strain KMN-mediated synthesis of AuNPs is rapid and in situ with antibiofilm and cytotoxicity activities. Moreover, it has the potential for an effective antibiofilm and cytotoxic activity that could be used in future therapeutic applications.



中文翻译:

使用放线菌 Amycolatopsis sp 的细胞外生物合成金纳米粒子的抗菌膜和细胞毒性潜力。国民党

摘要

本研究旨在使用Amycolatopsis sp. 生物合成金纳米粒子 (AuNPs)。KMN 并研究其潜在的抗生物膜、细胞毒性和抗氧化活性。通过紫外-可见光、能量色散 X 射线和傅里叶变换红外光谱,以及高分辨率透射电子显微镜、X 射线衍射、zeta 电位和动态光散射方法,确定了生物合成 AuNP 的物理化学特性。结晶紫测定和扫描电子显微镜表明,粒径为 44.4 nm 的 AuNPs 具有很强的抗生物膜活性(在 750 µg/ml 浓度下)对细菌菌株即金黄色葡萄球菌 ATCC 29213、大肠杆菌ATCC 25922铜绿假单胞菌ATCC 27853。结果还证明了对两种细胞系 MCF-7 和 HT-29 的强细胞毒活性。MTT 测试结果显示,在 48 小时内,AuNPs 的 IC 50对于 MCF-7 和 HT-29 细胞系分别为 600 和 300 µg/ml。AuNPs 对 DPPH 的IC 50为 46.87 µg/ml。这是第一份检查Amycolatopsis sp的报告。菌株 KMN 介导的 AuNPs 合成快速且原位具有抗生物膜和细胞毒性活性。此外,它具有有效的抗生物膜和细胞毒活性的潜力,可用于未来的治疗应用。

更新日期:2022-05-20
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