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Cyanine dye-assembled composite upconversion nanoparticles for the sensing and cell imaging of nitrite based on a single particle imaging method
Analyst ( IF 4.2 ) Pub Date : 2022-05-19 , DOI: 10.1039/d2an00594h
Yunchun Liu 1 , Wanru Zhu 1 , Xinru Wei 1 , Lun Wang 1 , Hongqi Chen 1
Affiliation  

In this work, a single particle imaging method based on the total internal reflection (TIR) imaging platform for the sensing and cell imaging of nitrite (NO2) in the near-infrared region using cyanine dye-assembled composite upconversion nanoparticles (UCNPs) was developed. The NaYF4:Yb,Tm@NaGdF4 UCNPs were synthesized as energy donors, and the cyanine dye IR-798 was prepared as an energy acceptor. Since the absorption spectrum of the cyanine dye IR-798 and the luminescence spectrum of upconversion nanoparticles overlapped effectively, IR-798 quenched the luminescence of the UCNPs. When NO2 was added to the cyanine dye-assembled composite upconversion nanoparticle system, NO2 destroyed the conjugate structure of IR-798, so that the luminous intensity of UCNPs could be restored. Based on the mechanism, a quantitative image analysis with high sensitivity, low sample usage, and fast response time using the TIR single particle imaging platform was developed to determine the content of nitrite in human serum samples. In addition, the UCNPs-IR-798 probe was applied to image the exogenous NO2 content in HeLa living cells based on the single particle imaging platform.

中文翻译:

用于基于单粒子成像方法的亚硝酸盐传感和细胞成像的花青染料组装复合上转换纳米粒子

在这项工作中,一种基于全内反射(TIR)成像平台的单粒子成像方法,用于使用花青染料组装的复合上转换纳米粒子(UCNPs)在近红外区域对亚硝酸盐(NO 2 - )进行传感和细胞成像发展了。合成了NaYF 4 :Yb,Tm@NaGdF 4 UCNPs作为能量供体,并制备了花青染料IR-798作为能量受体。由于花青染料 IR-798 的吸收光谱和上转换纳米粒子的发光光谱有效重叠,IR-798 淬灭了 UCNPs 的发光。当将NO 2 -添加到花青染料组装的复合上转换纳米粒子系统中时,NO 2 -破坏了IR-798的共轭结构,从而恢复了UCNPs的发光强度。基于该机理,利用TIR单粒子成像平台开发了一种高灵敏度、低样品使用量和快速响应时间的定量图像分析方法,用于测定人血清样品中亚硝酸盐的含量。此外,应用UCNPs-IR-798探针基于单粒子成像平台对HeLa活细胞中的外源NO 2 -含量进行成像。
更新日期:2022-05-24
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