当前位置: X-MOL 学术The Journal of Cell Biology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Filamin FLN-2 promotes MVB biogenesis by mediating vesicle docking on the actin cytoskeleton
The Journal of Cell Biology Pub Date : 2022-05-16 , DOI: 10.1083/jcb.202201020
Leiling Shi 1, 2, 3 , Youli Jian 1 , Meijiao Li 4 , Tianchao Hao 1 , Chonglin Yang 4 , Xiaochen Wang 1, 5
Affiliation  

Multivesicular bodies (MVBs) contain intralumenal vesicles that are delivered to lysosomes for degradation or released extracellularly for intercellular signaling. Here, we identified Caenorhabditis elegans filamin FLN-2 as a novel regulator of MVB biogenesis. FLN-2 co-localizes with V-ATPase subunits on MVBs, and the loss of FLN-2 affects MVB biogenesis, reducing the number of MVBs in C. elegans hypodermis. FLN-2 associates with actin filaments and is required for F-actin organization. Like fln-2(lf) mutation, inactivation of the V0 or V1 sector of V-ATPase or inhibition of actin polymerization impairs MVB biogenesis. Super-resolution imaging shows that FLN-2 docks V-ATPase-decorated MVBs onto actin filaments. FLN-2 interacts via its calponin-homology domains with F-actin and the V1-E subunit, VHA-8. Our data suggest that FLN-2 mediates the docking of MVBs on the actin cytoskeleton, which is required for MVB biogenesis.

中文翻译:

Filamin FLN-2 通过介导囊泡停靠在肌动蛋白细胞骨架上促进 MVB 生物发生

多泡体 (MVB) 包含腔内囊泡,这些囊泡被递送到溶酶体进行降解或释放到细胞外以进行细胞间信号传导。在这里,我们确定秀丽隐杆线虫细丝蛋白 FLN-2 是 MVB 生物发生的新型调节剂。FLN-2 与 MVB 上的 V-ATP 酶亚基共定位,FLN-2 的缺失会影响 MVB 的生物发生,从而减少线虫皮下组织中 MVB 的数量。FLN-2 与肌动蛋白丝结合,是 F-肌动蛋白组织所必需的。与 fln-2(lf) 突变一样,V-ATP 酶 V0 或 V1 部分的失活或肌动蛋白聚合的抑制会损害 MVB 生物合成。超分辨率成像显示 FLN-2 将 V-ATPase 修饰的 MVB 停靠在肌动蛋白丝上。FLN-2 通过其钙调蛋白同源结构域与 F-肌动蛋白和 V1-E 亚基 VHA-8 相互作用。我们的数据表明 FLN-2 介导 MVB 在肌动蛋白细胞骨架上的对接,这是 MVB 生物发生所必需的。
更新日期:2022-05-16
down
wechat
bug