Currently, there is insufficient viricide to effectively control tomato spotted wilt virus (TSWV). To address this pending issue, a series of thienopyrimidine-containing dithioacetal derivatives were prepared and tested for their anti-TSWV activities. A subsequent three-dimensional quantitative structure–activity relationship was constructed to indicate the development of optimal compound 35. The obtained compound 35 had excellent anti-TSWV curative, protective, and inactivating activities (63.0, 56.6, and 74.1%, respectively), and the EC₅₀ values of protective and inactivating activities of compound 35 were 252.8 and 113.5 mg/L, respectively, better than those of ningnanmycin (284.8 and 144.7 mg/L) and xiangcaoliusuobingmi (624.9 and 300.0 mg/L). In addition, the anti-TSWV activity of compound 35 was associated with defense-related enzyme activities, enhanced photosynthesis, and reduced stress response, thereby enhancing disease resistance.

中文翻译：

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含噻吩并嘧啶的二硫缩醛衍生物的设计、合成、抗番茄斑枯病病毒活性和作用机制

目前，没有足够的杀病毒剂来有效控制番茄斑枯病病毒（TSWV）。为了解决这个悬而未决的问题，制备了一系列含噻吩并嘧啶的二硫缩醛衍生物，并测试了它们的抗 TSWV 活性。随后构建了一个三维定量构效关系，以指示最佳化合物35的开发。得到的化合物35具有优异的抗TSWV治疗、保护和灭活活性（分别为63.0、56.6和74.1%），化合物35的保护和灭活活性的EC ₅₀值分别为 252.8 和 113.5 mg/L，优于宁南霉素（284.8 和 144.7 mg/L）和香草六索冰米（624.9 和 300.0 mg/L）。此外，化合物35的抗 TSWV 活性与防御相关酶活性、增强光合作用和降低应激反应有关，从而增强抗病性。