Lipoxygenase (LOX) pathway is the key and the most important pathway for the metabolism of fruit volatile. However, the transcription mechanism of the LOX pathway is still obscure in non-climacteric fruit. In order to identify the transcription factor involved in LOX-derived volatile metabolism, RNA sequencing and metabolome were used to profile strawberry (Fragaria × ananassa Duch.). Correlation analysis shows that the expression of FaLOX2 was negatively correlated with the hexanal content (r = −0.985). The RY element that specifically bound by the B3 domain transcription factors, are found in FaLOX2 promoter. B3 domain-containing transcription factors including LEAFY COTYLEDON2 (LEC2)–ABI3–VAL (LAV, 3), RELATED TO ABI3 and VP1 (RAV, 18), AUXIN RESPONSE FACTOR (ARF, 13), and REPRODUCTIVE MERISTEM (REM, 9) are identified. It is noticed that FaLEC2 expression is negatively correlated with (E,Z)-2,6-nonadienal content (r = −1.000). Yeast one-hybrid analysis and dual-luciferase analysis suggest that FaLEC2 transcriptionally represses the activity of FaLOX2 promoter. Moreover, FaLEC2 expression level is negatively correlated with FaLOX2 expression (r = −0.956). Here, our demonstration that FaLEC2 trans-represses the activity of the FaLOX2 promoter provides evidence that FaLEC2 directly controls a transcriptional program for LOX-derived volatile metabolism in strawberries.

脂氧合酶（LOX）途径是果实挥发物代谢的关键和最重要的途径。然而，LOX途径的转录机制在非更年期果实中仍不清楚。为了鉴定参与 LOX 衍生的挥发性代谢的转录因子，使用 RNA 测序和代谢组对草莓 ( Fragaria × ananassa Duch.) 进行分析。相关分析表明，FaLOX2的表达与己醛含量呈负相关（r  = -0.985）。在FaLOX2中发现了与 B3 结构域转录因子特异性结合的 RY 元件发起人。包含 B3 结构域的转录因子，包括 LEAFY COTYLEDON2 (LEC2)–ABI3–VAL (LAV, 3)、与 ABI3 和 VP1 相关的 (RAV, 18)、生长素反应因子 (ARF, 13) 和生殖分生组织 (REM, 9)被识别。注意到FaLEC2表达与 ( E,Z )-2,6-nonadienal 含量 ( r  = -1.000) 呈负相关。酵母单杂交分析和双荧光素酶分析表明FaLEC2在转录上抑制了FaLOX2启动子的活性。此外，FaLEC2表达水平与FaLOX2表达呈负相关（r  = -0.956）。在这里，我们演示FaLEC2反式抑制FaLOX2启动子的活性提供了FaLEC2直接控制草莓中 LOX 衍生的挥发性代谢的转录程序的证据。