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Simultaneous electrochemical immunosensing of relevant cytokines to diagnose and track cancer and autoimmune diseases
Bioelectrochemistry ( IF 5 ) Pub Date : 2022-05-14 , DOI: 10.1016/j.bioelechem.2022.108157
B Arévalo 1 , M Blázquez-García 1 , A Valverde 1 , V Serafín 1 , A Montero-Calle 2 , G Solís-Fernández 2 , R Barderas 2 , P Yáñez-Sedeño 1 , S Campuzano 1 , J M Pingarrón 1
Affiliation  

This work reports the first dual magnetic beads (MBs) assisted immunoplatform for the simultaneous determination of BAFF (B cell activation factor) and APRIL (a proliferation-induced ligand), two cytokines related to immunity and tumour invasion, growth and metastasis. The electrochemical immunoplatform involves sandwich-type immunoassays implemented on magnetic microparticles functionalized with neutravidin (NA-MBs) or carboxylic groups (HOOC-MBs), and amperometric detection (Eapp = − 0.20 V vs. Ag pseudo-reference electrode) at screen-printed dual carbon electrodes (SPdCE) using the H2O2/hydroquinone (HQ) system. The developed immunosensors provide improved sensitivity, with LOD values of 0.33 and 16.4 pg mL−1 for BAFF and APRIL, respectively, and much shorter assay time that those claimed for ELISA kits and allow their simultaneous determination. The dual immunosensor permits discrimination between healthy individuals and patients diagnosed with Systemic Lupus Erythematosus (SLE) or colorectal cancer (CRC) through the determination of both cytokines in 100-times diluted human sera with results in agreement with those provided by the individual ELISA methodologies.



中文翻译:

相关细胞因子的同时电化学免疫传感以诊断和追踪癌症和自身免疫性疾病

这项工作报告了第一个双磁珠 (MBs) 辅助免疫平台,用于同时测定 BAFF(B 细胞活化因子)和 APRIL(增殖诱导配体),这两种细胞因子与免疫和肿瘤侵袭、生长和转移有关。电化学免疫平台包括在用中性亲和素 (NA-MBs) 或羧基 (HOOC-MBs) 功能化的磁性微粒上实施的夹心型免疫测定,以及屏幕上的电流检测(E app  = - 0.20 V vs. Ag 伪参比电极)。使用 H 2 O 2 /对苯二酚 (HQ) 系统印刷双碳电极 (SPdCE) 。开发的免疫传感器提供更高的灵敏度,LOD 值为 0.33 和 16.4 pg mL -1分别用于 BAFF 和 APRIL,并且比 ELISA 试剂盒声称的检测时间短得多,并且允许同时测定。双免疫传感器允许通过测定 100 倍稀释的人血清中的两种细胞因子来区分健康个体和诊断为系统性红斑狼疮 (SLE) 或结肠直肠癌 (CRC) 的患者,结果与个体 ELISA 方法提供的结果一致。

更新日期:2022-05-18
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