A novel and simple turn-on fluorescence probe (HD) for Al³⁺ detection was successfully developed based on flavonol derivatives. This probe exhibited a significantly enhanced fluorescence response toward Al³⁺ in aqueous solution which could be observed by naked-eye from poor fluorescence to strong light green emission. The probe HD displays highly specific detection for Al³⁺ over other competitive metal ions, and the detection limit of probe HD for Al³⁺ was determined to be 2.57 × 10⁻⁸ M, which are much lower than the World Health Organization (WHO) guideline value for drinking food/water. The binding stoichiometry of probe HD with Al³⁺ was determined to be 1:1 according to Job’s plot and ESI-HRMS analysis, and the binding constant was calculated to be 2.01 × 10⁴ M⁻¹. The probe HD exhibited high selectivity, high sensitivity, good anti-interface ability, and wide pH application range as well as the quantitative determination in the detection of Al³⁺. The coordination mechanism of probe HD with Al³⁺ was supported by density functional theory (DFT) calculations and HRMS analysis. In addition, the probe HD was found to have good cell permeability and could be applied for live-cell imaging to detect Al³⁺ in onions and zebrafish.

基于黄酮醇衍生物，成功开发了一种用于Al ³⁺检测的新型、简单的开启荧光探针（HD） 。该探针在水溶液中对Al ³⁺表现出显着增强的荧光响应，肉眼可以观察到从弱荧光到强绿光发射。探针HD对Al ^{3+显示出比其他竞争性金属离子更高的特异性检测，探针}HD对Al ³⁺的检测限为2.57×10 ^-8 M，远低于世界卫生组织（WHO） ) 饮用食物/水的准则值。探针的结合化学计量根据Job's plot和ESI-HRMS分析确定HD与Al ³⁺为1:1，结合常数计算为2.01×10 ⁴ M ^-1。该探针HD在Al ³⁺的检测中表现出高选择性、高灵敏度、良好的抗界面能力、较宽的pH适用范围和定量测定等特点。密度泛函理论（DFT）计算和HRMS分析支持探针HD与Al ³⁺的配位机制。此外，发现探针HD具有良好的细胞渗透性，可用于活细胞成像检测Al ³⁺在洋葱和斑马鱼中。