Nuclear magnetic resonance (NMR) was applied to identify the metabolic differences in trehalose-treated and -untreated peach fruit during storage at 5°C in this study. Forty-one metabolites were identified using NMR; trehalose-treated peaches had higher sucrose, proline, and choline content, and lower glucose, fructose and gluconic acid content. These metabolic changes were accompanied by lower levels of chilling injury (CI). Trehalose-treated fruit had lower levels PpSnRK1 expression, resulting in lower sucrose decomposition and higher sucrose synthesis. We suggest that accumulated sucrose in trehalose-treated peaches is associated with the decrease in expression of PpSnRK1 and increases in the expression and activity of the sucrose phosphate synthase (SPS). This probably results in the improvement of chilling resistance in peach fruit.

在本研究中，应用核磁共振 (NMR) 来确定海藻糖处理和未处理的桃果实在 5°C 储存期间的代谢差异。使用 NMR 鉴定了 41 种代谢物；海藻糖处理的桃子的蔗糖、脯氨酸和胆碱含量较高，而葡萄糖、果糖和葡萄糖酸含量较低。这些代谢变化伴随着较低水平的冷害（CI）。海藻糖处理的果实具有较低水平的 PpSnRK1表达，导致较低的蔗糖分解和较高的蔗糖合成。我们认为海藻糖处理的桃子中积累的蔗糖与PpSnRK1表达的降低有关并增加蔗糖磷酸合酶 (SPS) 的表达和活性。这可能导致桃果实抗寒性的提高。