To explore the effect of ferulic acid (FA) on growth and nonspecific immunity of Macrobrachium nipponense, six experimental diets were supplemented with 0 (FA0), 20 mg/kg (FA20), 40 mg/Kg (FA40), 80 mg/kg (FA80) 160 mg/kg (FA160), 320 mg/kg (FA320) ferulic acid, respectively. M. nipponense (initial weight: 0.140 ± 0.015 g) was randomly assigned into 6 groups with 6 replicates for 10 weeks. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of FA80 and FA160 were significantly higher than those of the control group, and the feed conversion ratio (FCR) of FA80, FA160, and FA320 group was significantly lower than that of the control group. Compared with the control group, the albumin/globulin ratio (A/G) of FA160 and FA320 group, and the complement 3 (C3) of all the treat groups was significantly increased. The glucose (GLU) of FA40 was significantly higher than that of other groups. Compared with the control group, aspartate aminotransferase (AST), inducible nitric oxide synthase (iNOS), nitric oxide (NO) and malondialdehyde (MDA) in the FA80 and FA160 group were significantly reduced, the superoxide dismutase (SOD) in the FA160 group and the glutathione peroxidase (GPX) in the FA80 and FA160 groups were significantly increased. The FA160 group significantly down-regulated the expression of Toll and Dorsal, immune deficiency (IMD). Relish of the FA80 and FA160 group was significantly down-regulated, and heat shock protein (HSP70) of the FA40, FA80 and FA160 group was significantly down-regulated compared with the control. Results of the infection test showed that FA160 group enhanced resistance against non-O1 Vibrio cholera GXFL1-X infection in M. nipponense. In conclusion, we performed a polynomial comparison with WGR, SGR, and FCR and obtained the optimal amount of FA with 163.99–183.33 mg/kg, which could significantly improve the growth, non-specific immunity and disease resistance of M. nipponense.

为探讨阿魏酸（FA）对日本沼虾生长和非特异性免疫的影响，在6个实验日粮中分别添加了0（FA0）、20 mg/kg（FA20）、40 mg/Kg（FA40）、80 mg/kg (FA80) 160 mg/kg (FA160)、320 mg/kg (FA320) 阿魏酸。M. nipponense（初始重量：0.140 ± 0.015 g）随机分为 6 组，6 次重复，持续 10 周。结果表明，FA80和FA160的增重率（WGR）和比生长率（SGR）显着高于对照组，FA80、FA160和FA320组的饲料转化率（FCR）显着高于对照组。低于对照组。与对照组相比，FA160和FA320组的白蛋白/球蛋白比值（A/G）以及所有治疗组的补体3（C3）均显着升高。FA40的葡萄糖（GLU）显着高于其他组。与对照组相比，FA80和FA160组的天冬氨酸氨基转移酶（AST）、诱导型一氧化氮合酶（iNOS）、一氧化氮（NO）和丙二醛（MDA）显着降低，FA160组的超氧化物歧化酶（SOD）和FA80和FA160组的谷胱甘肽过氧化物酶（GPX）显着升高。FA160 组显着下调Toll和Dorsal，免疫缺陷 ( IMD )。与对照组相比，FA80、FA160组的津津有味显着下调，FA40、FA80和FA160组的热休克蛋白（HSP70）显着下调。感染试验结果表明，FA160组增强了日本分枝杆菌对非O1霍乱弧菌GXFL1-X感染的抵抗力。综上所述，我们对WGR、SGR和FCR进行了多项式比较，得出了FA的最佳用量为163.99-183.33 mg/kg，可以显着提高日本分枝杆菌的生长、非特异性免疫和抗病性。