In this study, a pH-responsive magnetic drug delivery system was prepared by cross-linking of Cofe₂O₄/graphene oxide to carboxymethyl cellulose (CMC) biopolymer with adipic acid dihydrazide (ADH) and then investigated for loading and release of a poorly water-soluble anti-cancer drug, curcumin (CUR). The CoFe₂O₄/GO-ADH-CMC bio-nanocomposite was characterized by FE–SEM/EDX, TEM, FT–IR, XRD, TGA, VSM, and zeta-potential techniques. The highest amount of drug loading was achieved as 23.7 mg g^–1 at 323 K, pH 6.0, and 120 mg L^–1 CUR concentration. The study of adsorption isotherms revealed that the Sips model was best fitted to the equilibrium data (R² > 0.997 and RMSE < 0.48), which indicated the contribution of both chemisorption and physisorption in drug loading. Furthermore, the release profile of CUR from drug-loaded CoFe₂O₄/GO-ADH-CMC was studied in mimicking the endosomal (pH = 5.6) and physiological (pH = 7.4) conditions. After 24 h, the maximum cumulative releases were found to be 86 and 38% at pH = 5.6 and 7.4, respectively. Also, the Peppas-Sahlin kinetic model was followed for the release data. The MTT assay exhibited good biocompatibility of the CoFe₂O₄/GO-ADH-CMC. The results implied that CoFe₂O₄/GO-ADH-CMC magnetic bio-nanocomposite might be utilized as a potentially safe carrier with the continuous and slow release of CUR.

Graphical Abstract

中文翻译：

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基于羧甲基纤维素胺化氧化石墨烯的 pH 响应磁性纳米载体的制备用于姜黄素的负载和体外释放

_{在本研究中，通过将 Cofe 2} O ₄ /氧化石墨烯与羧甲基纤维素 (CMC) 生物聚合物与己二酸二酰肼 (ADH)交联，制备了一种 pH 响应的磁性药物递送系统，然后研究了其负载和释放的不良反应。水溶性抗癌药姜黄素（CUR）。CoFe ₂ O ₄ /GO-ADH-CMC 生物纳米复合材料通过 FE-SEM/EDX、TEM、FT-IR、XRD、TGA、VSM 和 zeta 电位技术进行了表征。在 323 K、pH 6.0 和 120 mg L ^–1 CUR 浓度下，最高载药量为 23.7 mg g ^{–1 。}吸附等温线的研究表明，Sips 模型最适合平衡数据（R ² > 0.997 和 RMSE < 0.48），这表明化学吸附和物理吸附对载药量的贡献。此外，在模拟内体 (pH = 5.6) 和生理 (pH = 7.4) 条件下研究了载药 CoFe ₂ O ₄ /GO-ADH-CMC中 CUR 的释放曲线。24 小时后，在 pH = 5.6 和 7.4 时，最大累积释放量分别为 86% 和 38%。此外，释放数据遵循 Peppas-Sahlin 动力学模型。MTT测定显示CoFe ₂ O ₄ /GO-ADH-CMC良好的生物相容性。结果表明，CoFe ₂ O ₄/GO-ADH-CMC 磁性生物纳米复合材料可用作具有持续和缓慢释放 CUR 的潜在安全载体。

图形概要