In recent years, the colon has become a hot topic in biopharmaceutical research as several in vitro models of the human colon have been presented. A major focus is on the characterization of the microbiota and its capabilities. The aim of the present study was to further develop the MimiCol, preserving its properties and accelerating data acquisition. Emphasis was placed on the simplicity of its design and easy scalability. To prove the viability of the concept, degradation of sulfasalazine was investigated, and the bacterial composition during the experiment was assessed by 16S rRNA sequencing. The transfer of the experimental conditions to the new model was successful. Commercially available components were implemented in the setup. The model MimiCol³ represented the colon ascendens satisfactorily in its properties regarding volume, pH value, and redox potential. 16S rRNA sequencing led to further insights into the bacterial composition in the vessels. Degradation of sulfasalazine was in good agreement with in vivo data. The new model of the colon ascendens MimiCol³ enabled us to collect more reliable data, as three experiments were conducted simultaneously under the same conditions.

中文翻译：

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模拟人体升结肠动态生理条件的先进生物反应器：MimiCol3

近年来，随着人体结肠体外模型的出现，结肠已成为生物制药研究的热门话题。一个主要关注点是微生物群及其能力的表征。本研究的目的是进一步开发 MimiCol，保留其特性并加速数据采集。重点放在其设计的简单性和易于扩展性上。为了证明这一概念的可行性，研究了柳氮磺吡啶的降解，并通过 16S rRNA 测序评估了实验过程中的细菌组成。将实验条件转移到新模型是成功的。商业上可用的组件在设置中实现。模型 MimiCol ³在体积、pH 值和氧化还原电位方面的特性令人满意地代表了升结肠。16S rRNA 测序有助于进一步了解血管中的细菌组成。柳氮磺吡啶的降解与体内数据非常吻合。升结肠 MimiCol ³的新模型使我们能够收集更可靠的数据，因为在相同条件下同时进行了三个实验。