The sequential bio-treatment of refractory carbonaceous gold ore is a promising solution to recover gold effectively by environmentally friendly technology, which includes bio-oxidation of sulfide and biodegradation of carbonaceous matter by lignin-degrading enzymes. There are several drawbacks in enzyme treatment using cell-free spent medium (CFSM), including lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium, in particular the poor stability of enzyme activities. In the present work, laccase-mediator system (LMS) was applied for the degradation of carbonaceous matter in real gold ore to improve the efficiency of gold extraction as well as handling. The LMS was intended to be a great alternative process of CFSM with utilizing purified laccase in the presence of 1-hydroxybenzotriazole as a mediator. The application of LMS provided several advantages including not only greater stability, greater efficiency to degrade carbonaceous matter, better handling, much saving the treatment time, but also wider availability in laccase. In addition, replacing bio-oxidation with ferric chloride leaching as the dissolution path of sulfides facilitated avoiding the formation of jarosite and saving the required time. The gold recovery by cyanidation was improved from 41.5 ± 0.3% for the starting material to 81.3 ± 3.9% (n = 2) for the solid residues after the modified sequential pretreatment. This is correspondent to 86.3% of gold recovery for the extractable maximum gold excluding the enclosed gold in acid-insoluble silicates. The improved process involving LMS can be proposed with valuable advantages to fit a sustainable metallurgical technology of gold ores.

难处理碳质金矿石的序贯生物处理是一种通过环保技术有效回收黄金的有前景的解决方案，包括硫化物的生物氧化和木质素降解酶对碳质物质的生物降解。使用无细胞废培养基 (CFSM) 进行酶处理有几个缺点，包括来自Phanerochaete chrysosporium 的木质素过氧化物酶和锰过氧化物酶，特别是酶活性的稳定性差。在目前的工作中，漆酶介体系统（LMS）被应用于降解真金矿石中的含碳物质，以提高黄金提取和处理的效率。LMS 旨在成为 CFSM 的一个很好的替代工艺，在 1-羟基苯并三唑作为介体的情况下使用纯化的漆酶。LMS 的应用提供了几个优点，不仅包括更高的稳定性、更高的含碳物质降解效率、更好的处理、大大节省了处理时间，而且在漆酶中更广泛的可用性。此外，用三氯化铁浸出代替生物氧化作为硫化物的溶解路径有助于避免黄钾铁矾的形成并节省所需时间。氰化金回收率从 41 提高。n  = 2) 用于改进的顺序预处理后的固体残留物。这相当于不包括酸不溶性硅酸盐中封闭金的可提取最大金的金回收率为 86.3%。可以提出涉及 LMS 的改进工艺，具有宝贵的优势，以适应金矿石的可持续冶金技术。