In order to accelerate the application of ABPs in shrimp and crab feed, we evaluated the effect of ABPs on the growth, non-specific immunity and disease resistance of crayfish. Crayfish (Procambarus clarkii) were randomly divided into six groups, the fed diets supplemented with different levels of ABPs: 0, 0.05, 0.15, 0.3, 0.45 and 0.6 g/kg. After 28 days of feeding, ABPs were found to significantly increase FBW and WG of crayfish. The addition of 0.15–0.6 g/kg of ABPs to the feed significantly increased the activities of ACP, AKP and T-SOD, decreased the activity of MDA enzymes in the hepatopancreas of crayfish. In the hemolymph, ABPs at 0.3–0.45 g/kg can significantly increase the activities of ACP, AKP, T-SOD and LZM enzymes, while the addition of 0.15 or 0.6 g/kg has no significant difference with the control group. The results of qPCR showed the addition of 0.15–0.6 g/kg ABPs to the feed could significantly up-regulate the expression levels of gpx, lec and hsp70 mRNA in the hepatopancreas (P < 0.05). Adding 0.15–0.6 g/kg of ABPs could up-regulate the expression of ctsb and lec gene in gills. In addition, the expression of alf was up-regulated in gills only when the addition amount of ABPs was 0.3 g/kg. While the expression of mtMnsod in gills was up-regulated when the addition amount was 0.3–0.6 g/kg. Addition of 0.05–0.6 g/kg of ABPs reduced the mortality of crayfish infected by A.hydrophila. The above results indicated that the addition of ABPs could improve the growth, non-specific immunity and disease resistance of crayfish, and the addition of 0.3 g/kg and 0.45 g/kg of ABPs might be beneficial to the culture of crayfish.

为了加快ABPs在虾蟹饲料中的应用，我们评估了ABPs对小龙虾生长、非特异性免疫和抗病能力的影响。小龙虾 ( Procambarus clarkii) 被随机分为 6 组，饲喂的日粮中添加了不同水平的 ABP：0、0.05、0.15、0.3、0.45 和 0.6 g/kg。饲喂 28 天后，发现 ABP 显着增加了小龙虾的 FBW 和 WG。在饲料中添加0.15-0.6 g/kg ABPs显着提高了ACP、AKP和T-SOD的活性，降低了小龙虾肝胰腺中MDA酶的活性。在血淋巴中，0.3~0.45 g/kg的ABPs可显着提高ACP、AKP、T-SOD和LZM酶的活性，而添加0.15或0.6 g/kg与对照组无显着差异。qPCR结果显示，饲料中添加0.15~0.6 g/kg ABPs可显着上调肝胰腺中gpx、lec和hsp70 mRNA的表达水平（P <0.05)。添加0.15~0.6 g/kg的ABPs可上调鳃中ctsb和lec基因的表达。此外，仅当ABPs的添加量为0.3 g/kg时，鳃中alf的表达才上调。而当添加量为0.3~0.6 g/kg时，鳃中mtMnsod的表达上调。添加 0.05–0.6 g/kg ABPs 可降低感染A.hydrophila的小龙虾的死亡率。上述结果表明，添加ABPs可以提高小龙虾的生长、非特异性免疫和抗病能力，添加0.3 g/kg和0.45 g/kg的ABPs可能有利于小龙虾的养殖。