In this study, the acceleratory effect of magnesium oxide nanoparticles (MgO NPs) on the amyloid fibrillization of human tau protein, a major protein involved in the onset of Alzheimer’s disease (AD) was investigated. The MgO NPs were fabricated through laser ablation synthesis in solution (LASiS), well-characterized, and explored further for tau aggregation and relevant neurotoxicity by different assays. The results showed that the MgO NPs have a size of around 30 nm, a hydrodynamic radius of 57.09 nm, and a zeta potential of −18.06 mV. The data from ThT and ANS fluorescence-based assays along with circular dichroism (CD) spectroscopy clearly indicated that MgO NPs could significantly promote tau fibrillization, concentration-dependently. Considering the acceleratory effect of MgO NPs against tau fibrillization, cellular assays including cell viability, reactive oxygen species (ROS), and caspase-3 assays indicated that the neurotoxicity of tau amyloid fibrils formed with MgO NPs was higher than that of tau samples aged alone against N2a neuron-like cells. Therefore, it was concluded that the interaction of MgO NPs with tau can lead to acceleration of tau aggregation and underlying neurotoxicity. This study, then can provide useful information about the direct effect of MgO NPs against memory proteins and subsequent adverse effects.

在这项研究中，研究了氧化镁纳米粒子 (MgO NPs) 对人类 tau 蛋白淀粉样蛋白原纤维化的促进作用，该蛋白是参与阿尔茨海默病 (AD) 发病的主要蛋白质。MgO NPs 是通过溶液中的激光烧蚀合成 (LASiS) 制造的，经过充分表征，并通过不同的测定进一步探索了 tau 聚集和相关的神经毒性。结果表明，MgO NPs 的尺寸约为 30 nm，流体动力学半径为 57.09 nm，zeta 电位为 -18.06 mV。来自 ThT 和 ANS 基于荧光的测定以及圆二色性 (CD) 光谱的数据清楚地表明，MgO NPs 可以显着促进 tau 纤维化，浓度依赖性。考虑到 MgO NPs 对 tau 纤维化的促进作用，包括细胞活力、活性氧 (ROS) 和 caspase-3 测定在内的细胞测定表明，与 MgO NPs 形成的 tau 淀粉样蛋白原纤维的神经毒性高于单独老化的 tau 样品对 N2a 神经元样细胞的神经毒性。因此，得出的结论是，MgO NPs 与 tau 的相互作用可导致 tau 聚集加速和潜在的神经毒性。然后，这项研究可以提供有关 MgO NPs 对记忆蛋白的直接影响和随后的不利影响的有用信息。得出的结论是，MgO NPs 与 tau 的相互作用可导致 tau 聚集加速和潜在的神经毒性。然后，这项研究可以提供有关 MgO NPs 对记忆蛋白的直接影响和随后的不利影响的有用信息。得出的结论是，MgO NPs 与 tau 的相互作用可导致 tau 聚集加速和潜在的神经毒性。然后，这项研究可以提供有关 MgO NPs 对记忆蛋白的直接影响和随后的不利影响的有用信息。