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Screen-printed carbon electrode modified with de-bundled single-walled carbon nanotubes for voltammetric determination of norepinephrine in ex vivo rat tissue
Bioelectrochemistry ( IF 5 ) Pub Date : 2022-05-10 , DOI: 10.1016/j.bioelechem.2022.108155
Thenmozhi Rajarathinam 1 , Dinakaran Thirumalai 1 , Minho Kwon 2 , Seulah Lee 3 , Sivaguru Jayaraman 1 , Hyun-Jong Paik 2 , Jaewon Lee 3 , Seung-Cheol Chang 1
Affiliation  

A voltammetric sensor for norepinephrine (NE) detection was developed by modifying a disposable screen-printed carbon electrode (SPCE) with de-bundled single-walled carbon nanotubes (D-SWCNTs). The de-bundling was carried out using a newly synthesized polymeric dispersant, a co-polymer of polystyrene sulfonate and methacrylate of lipoic acid. The D-SWCNTs/SPCE showed better sensitivity towards NE compared to the bare SPCE and that modified with bundled SWCNTs. The sensor was optimized for detecting NE by differential pulse voltammetry (DPV) in terms of the D-SWCNTs concentration, DPV parameters, and solution pH. Under the optimum conditions, the sensor exhibited a dynamic linear range of 100 nM–2.0 µM NE, and the detection limit was 62.0 nM (S/N = 3). Additionally, the effects of possible interferents were investigated. The relative standard deviation for five successive measurements of 2.0 µM NE was 7.6%, and approximately 75.8% of the sensor activity was retained after four weeks of storage. The practical potential of this sensor was demonstrated by quantifying NE in ex vivo rat tissue samples.



中文翻译:

用去束单壁碳纳米管修饰的丝网印刷碳电极用于伏安法测定离体大鼠组织中的去甲肾上腺素

一种用于去甲肾上腺素 (NE) 检测的伏安传感器是通过使用去捆绑的单壁碳纳米管 (D-SWCNT) 修改一次性丝网印刷碳电极 (SPCE) 开发的。使用新合成的聚合物分散剂、聚苯乙烯磺酸盐和硫辛酸甲基丙烯酸酯的共聚物进行解束。与裸 SPCE 和用捆绑 SWCNT 修饰的相比,D-SWCNT/SPCE 对 NE 表现出更好的敏感性。该传感器针对 D-SWCNT 浓度、DPV 参数和溶液 pH 值通过差分脉冲伏安法 (DPV) 检测 NE 进行了优化。在最佳条件下,传感器的动态线性范围为 100 nM–2.0 µM NE,检测限为 62.0 nM (S/N = 3)。此外,还研究了可能的干扰物的影响。5 次连续测量 2.0 µM NE 的相对标准偏差为 7.6%,存储 4 周后保留了大约 75.8% 的传感器活动。该传感器的实际潜力通过量化 NE 在离体大鼠组织样本。

更新日期:2022-05-13
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