Ultrasonics Sonochemistry ( IF 8.4 ) Pub Date : 2022-05-11 , DOI: 10.1016/j.ultsonch.2022.106032 Weiqing Lan 1 , Yuqing Sun 2 , Shucheng Liu 3 , Yuan Guan 2 , Shengyun Zhu 2 , Jing Xie 4
The effects of ultrasound-assisted chitosan grafted caffeic acid coating on the quality and microbial composition of fresh pompano (Trachinotus ovatus) fillets during ice storage for 24 days were evaluated. Samples were treated by distilled water (CK), ultrasound (US), chitosan grafted caffeic acid coating (G), and chitosan grafted caffeic acid coating with ultrasound-assisted (USG). Results showed that samples treated with USG could inhibit the formation of corrupt substances such as TVB-N, TBA, biogenic amines (BAs), hypoxanthine (Hx), and hypoxanthine riboside (HxR) when compared to the CK group. The results of high-throughput sequencing technology observed that the major bacteria genus of fresh samples was Acinetobacter. The diversity of bacterial communities at the initial stage was more diverse than that at the end of stage. With the extension of storage time, the USG treatment could maintain the microbial diversity. The dominant microbiota was Shewanella and Brochothrix in the CK group after 24 days of storage. In addition, Brochothrix in treated groups was effectively decreased. The microbial communities of samples in all treatments were changed during storage. At the end of storage, there was a significant difference in bacterial composition between the CK and treated samples, indicating that the treatment can effectively inhibit the growth of microorganisms, especially spoilage microorganisms, and reduce the quality deterioration caused by bacteria.
中文翻译:
超声辅助壳聚糖接枝咖啡酸涂层对冰贮期间鲳鱼品质及微生物组成的影响
评估了超声辅助壳聚糖接枝咖啡酸涂层对新鲜鲳鱼(Trachinotus ovatus)鱼片在冷藏24天期间质量和微生物组成的影响。通过蒸馏水 (CK)、超声 (US)、壳聚糖接枝咖啡酸涂层 (G) 和壳聚糖接枝咖啡酸涂层与超声辅助 (USG) 处理样品。结果表明,与 CK 组相比,经 USG 处理的样品可抑制 TVB-N、TBA、生物胺 (BAs)、次黄嘌呤 (Hx) 和次黄嘌呤核苷 (HxR) 等腐败物质的形成。 高通量测序技术结果观察到新鲜样品的主要菌属为不动杆菌属。 初始阶段细菌群落的多样性比阶段结束时更加多样化。随着储存时间的延长,USG处理可以保持微生物的多样性。储存 24 天后,CK 组的优势微生物群是希瓦氏菌属和布罗氏菌属。此外,治疗组中的Brochothrix有效减少。所有处理样品的微生物群落在储存过程中发生了变化。贮藏结束时,对照样品与处理样品的细菌组成存在显着差异,说明处理可有效抑制微生物特别是腐败微生物的生长,减少细菌引起的品质变质。