Novel polycaprolactone-based polyurethane (PCL-PU) copolymers with hyperelasticity, shape-memory, and ultra-cell-adhesion properties are reported as clinically applicable tissue-regenerative biomaterials. New isosorbide derivatives (propoxylated or ethoxylated ones) were developed to improve mechanical properties by enhanced reactivity in copolymer synthesis compared to the original isosorbide. Optimized PCL-PU with propoxylated isosorbide exhibited notable mechanical performance (50 MPa tensile strength and 1150% elongation with hyperelasticity under cyclic load). The shape-memory effect was also revealed in different forms (film, thread, and 3D scaffold) with 40%–80% recovery in tension or compression mode after plastic deformation. The ultra-cell-adhesive property was proven in various cell types which were reasoned to involve the heat shock protein-mediated integrin (α5 and αV) activation, as analyzed by RNA sequencing and inhibition tests. After the tissue regenerative potential (muscle and bone) was confirmed by the myogenic and osteogenic responses in vitro, biodegradability, compatible in vivo tissue response, and healing capacity were investigated with in vivo shape-memorable behavior. The currently exploited PCL-PU, with its multifunctional (hyperelastic, shape-memorable, ultra-cell-adhesive, and degradable) nature and biocompatibility, is considered a potential tissue-regenerative biomaterial, especially for minimally invasive surgery that requires small incisions to approach large defects with excellent regeneration capacity.

中文翻译：

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用于组织再生的超弹性、形状记忆和超细胞粘附性可降解聚己内酯-聚氨酯共聚物

据报道，具有超弹性、形状记忆和超细胞粘附特性的新型聚己内酯基聚氨酯 (PCL-PU) 共聚物可作为临床适用的组织再生生物材料。与原始异山梨醇相比，开发了新的异山梨醇衍生物（丙氧基化或乙氧基化的衍生物），通过增强共聚物合成中的反应性来改善机械性能。使用丙氧基化异山梨醇优化的 PCL-PU 表现出显着的机械性能（50 MPa 的拉伸强度和 1150% 的伸长率，在循环载荷下具有超弹性）。形状记忆效应也以不同的形式（薄膜、线和 3D 支架）显示，在塑性变形后的拉伸或压缩模式下恢复 40%–80%。通过 RNA 测序和抑制测试分析，在各种细胞类型中证明了超细胞粘附特性，这些细胞类型被认为涉及热休克蛋白介导的整合素（α5 和 αV）激活。在体外的生肌和成骨反应证实组织再生潜力（肌肉和骨骼）后、生物降解性、相容的体内组织反应和愈合能力与体内形状记忆行为进行了研究。目前开发的 PCL-PU 具有多功能（超弹性、形状记忆、超细胞粘附和可降解）性质和生物相容性，被认为是一种潜在的组织再生生物材料，特别是对于需要小切口的微创手术。具有优良再生能力的大缺陷。