Carcinoembryonic antigen (CEA) is a glycoprotein, one of the common tumor biomarkers, found at low levels in body fluids. Generally, overexpression of CEA is found in various cancers, including ovarian, breast, lung, colorectal, gastric, and pancreatic cancers. Since CEA is an important tumor biomarker, the quantification of CEA is helpful for diagnosing cancer, monitoring tumor progression, and the follow-up treatment. This research develops a highly sensitive sandwich aptasensor for CEA identification on an interdigitated electrode sensor. Carbon-based material was used to attach a higher anti-CEA capture aptamer onto the sensor surface through a chemical linker, and then, CEA was quantified by the aptamer. Furthermore, CEA-spiked serum was tested by using the immobilized aptamer, which was found to not affect the target validation. The limit of detection for CEA in PBS and serum is calculated from a linear regression graph to be 0.5 ng/mL with R² values of 0.9593 and 0.9657, respectively, over a linear range from 0.5 to 500 ng/mL. This CEA quantification by the aptasensor can help diagnose various surgical tumors and monitor their progression.

中文翻译：

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用于监测手术肿瘤的适配体传感器上的碳材料混合结构

癌胚抗原 (CEA) 是一种糖蛋白，是常见的肿瘤生物标志物之一，在体液中含量较低。通常，CEA 的过表达存在于各种癌症中，包括卵巢癌、乳腺癌、肺癌、结肠直肠癌、胃癌和胰腺癌。由于CEA是重要的肿瘤生物标志物，因此CEA的量化有助于癌症的诊断、肿瘤进展的监测和后续治疗。本研究开发了一种高度灵敏的夹层适体传感器，用于在叉指电极传感器上进行 CEA 识别。碳基材料用于通过化学接头将更高的抗CEA捕获适体连接到传感器表面，然后通过适体对CEA进行量化。此外，使用固定化适配体对 CEA 加标血清进行了测试，发现该适配体不会影响目标验证。在 0.5 至 500 ng/mL 的线性范围内， R ²值分别为 0.9593 和 0.9657。适体传感器的这种 CEA 量化可以帮助诊断各种手术肿瘤并监测它们的进展。