The Biotronic® Top3 (OCAP: organic acids, cinnamaldehyde, and permeabilising complex) is used for improving microbial community in monogastric animals. This research was aimed to test the five dosages of OCAP (0%: control, 0.05%: OCAP0.05, 0.10%: OCAP0.1, 0.15%: OCAP0.15%, and 0.20%: OCAP0.2) in barramundi (Lates calcarifer) (8.3 ± 0.3 g) for eight weeks. The results showed a linear relation between OCAP level and growth rate, feed conversion ratio, protease, lipase, lysozyme in serum and mucus, alternative complement activity (ACH50) in serum, catalase, glutathione peroxidase, and malondialdehyde levels (P < 0.05). Accordingly, the OCAP0.2 treatment had the best performance in the abovementioned parameters. While there was no significant difference in expression of heat shock proteins (HSP70, HSP90) and tumour necrosis factor (TNF-a) among groups, interleukins (IL-1β, IL-8) were significantly lower in the OCAP0.15 and OCAP0.2 group than others. The expression of IGF-1 followed the growth rate trend. Generally, feeding fish with the OCAP0.2 diet improved growth rate, digestive enzymes activities, immune response, antioxidant system, and gene expressions in barramundi. As the linear relation was observed, supplementing more levels of OCAP to reach a plateau is recommended.

Biotronic® Top3（OCAP：有机酸、肉桂醛和透化复合物）用于改善单胃动物的微生物群落。本研究旨在在澳洲肺鱼中测试五种剂量的 OCAP（0%：对照、0.05%：OCAP0.05、0.10%：OCAP0.1、0.15%：OCAP0.15% 和 0.20%：OCAP0.2）。Lates calcarifer ) (8.3 ± 0.3 g) 持续八周。结果表明，OCAP水平与生长速率、饲料转化率、蛋白酶、脂肪酶、血清和粘液中的溶菌酶、血清中的替代补体活性（ACH50）、过氧化氢酶、谷胱甘肽过氧化物酶和丙二醛水平呈线性关系（P < 0.05)。因此，OCAP0.2 处理在上述参数中表现最好。虽然各组间热休克蛋白（HSP70、HSP90）和肿瘤坏死因子（TNF-a）的表达无显着差异，但OCAP0.15和OCAP0中白细胞介素（IL-1β、IL-8）的表达明显降低。 2组比别人。IGF-1的表达遵循增长率趋势。一般来说，用 OCAP0.2 饲料喂养鱼可以提高澳洲肺鱼的生长速度、消化酶活性、免疫反应、抗氧化系统和基因表达。由于观察到线性关系，建议补充更多水平的 OCAP 以达到平稳状态。