In this study, we observed the activity of polyphenoloxidase (PPO) in red Fuji apples in the presence of single-chained surfactants (including cetyl trimethyl ammonium bromide (CTAB), and dodecyl trimethyl ammonium bromide (DTAB)) and gemini surfactants (pentamethylene-α,ω-bis(dodecyl dimethyl ammonium bromide), octamethylene-α,ω-bis(dodecyl dimethyl ammonium bromide, dodecamethylene-α,ω-bis(dodecyl dimethyl ammonium bromide), pentamethylene-α,ω-bis(cetyl dimethyl ammonium bromide), and octamethylene-α,ω-bis(cetyl dimethyl ammonium bromide)). It was found that all these surfactants enhanced the activity of PPO in a wide range of temperature at low content. When PPO was denatured by incubating at high temperature, the surfactants caused reactivation of PPO. Compared to the single-chained surfactants, the gemini surfactants increased PPO activity at a much lower concentration. Moreover, the single-chained surfactant and the gemini surfactant acted together to further increase PPO activity, and the synergistic effect reduced the amount of surfactant used. In addition, the Michaelis-Menten constant for PPO did not change in the presence of the surfactants, suggesting the active site should remain well with the surfactants.

中文翻译：

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阳离子表面活性剂促进红富士苹果多酚氧化酶的活性——表面活性剂结构的作用

在这项研究中，我们观察了多酚氧化酶（PPO）的活性红富士单链表面活性剂和双子表面活性剂共同作用进一步提高了PPO活性，协同作用减少了表面活性剂的用量。此外，PPO 的 Michaelis-Menten 常数在表面活性剂的存在下没有改变，表明活性位点应该与表面活性剂保持良好的关系。