In the present study, the lipase from Aspergillus fumigatus was purified which was found to be stable to commercial detergents and oxidising agents. A purification fold of 6.96 and yield of 11.03% were achieved when the enzyme was purified using Octyl Sepharose column chromatography. In presence of various oxidizing agents, the highest activity of lipase was 15.56 U/mg with hydrogen peroxide. Among various surfactants used, the maximum activity exhibited by lipase was with Tween 80. While studying the effect of various detergents, the highest activity of 9.3 U/mg was achieved with "Vanish" detergent. Wash performance was studied with various detergents out of which "Vanish" showed highest oil removal of 79%. Lipase from Aspergillus fumigatus possessed better stability with various surfactants and oxidizing agents. The results of this study have shown that the lipase from Aspergillus fumigatus along with detergent "Vanish" (0.7%) under optimized conditions (5 μg/ml lipase, 40°C wash temperature and 40 min wash duration) improved oil removal from cotton fabric stained with mustard oil by 84%.

中文翻译：

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一种从烟曲霉中纯化的耐碱性和耐洗涤剂的脂肪酶，在去除棉织物上的芥子油污渍方面具有潜在应用

在本研究中，脂肪酶来自烟曲霉纯化后发现对商业洗涤剂和氧化剂稳定。当使用辛基琼脂糖柱层析纯化酶时，获得了 6.96 的纯化倍数和 11.03% 的产率。在各种氧化剂存在下，脂肪酶与过氧化氢的最高活性为15.56 U/mg。在使用的各种表面活性剂中，脂肪酶表现出的最大活性是吐温8​​0。在研究各种洗涤剂的效果时，“Vanish”洗涤剂的最高活性达到了9.3 U/mg。用各种洗涤剂研究洗涤性能，其中“Vanish”显示出最高的 79% 的油去除率。脂肪酶来自烟曲霉对各种表面活性剂和氧化剂具有较好的稳定性。本研究结果表明，脂肪酶烟曲霉在优化条件（5 μg/ml 脂肪酶、40°C 洗涤温度和 40 分钟洗涤时间）下与洗涤剂“Vanish”（0.7%）一起，将沾有芥子油的棉织物的油去除率提高了 84%。