Greenhouse cultivation is mainly a monoculture system, which leads to severe soil degradation, with significant changes to the structure of the soil microbial community. The present study aimed to determine the effects of fertilizer and waterlogging (WL) on the microbial community of soil that was continuously monocropped with cucumber in a greenhouse for 3 years (2016–2019). Biolog EcoPlates™ and high-throughput sequencing were used to investigate the abundance, structure, and diversity of the soil microbial community. Compared with the initial total soluble salt content (8.65 g/kg), total soluble salt content after WL significantly decreased to 0.597 g/kg. Compared to control (CK) soil, the abundance of fungal species in the chemical fertilizer plus waterlogging (CFWL)-treated soil increased by 40.5%, while bacterial species abundance in the organic fertilizer plus waterlogging (OFWL)-treated soil increased by 10.7%. Furthermore, the relative abundance of Arthrobacter, Geobacter, and Bacillus was higher in OFWL-treated soil than CFWL-treated soil (p < 0.01). The application of CFWL to greenhouse soil under continuous monoculture produces fungal-dominated soil, whereas OFWL application alone produces bacterial-dominated soil.

中文翻译：

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施肥和涝渍对温室栽培土壤微生物群落多样性和功能的影响

温室栽培主要是单一栽培系统，导致土壤严重退化，土壤微生物群落结构发生显着变化。本研究旨在确定肥料和涝渍 (WL) 对在温室中连续种植黄瓜 3 年（2016-2019 年）的土壤微生物群落的影响。Biolog EcoPlates™ 和高通量测序用于研究土壤微生物群落的丰度、结构和多样性。与初始总可溶性盐含量（8.65 g/kg）相比，WL后总可溶性盐含量显着降低至0.597 g/kg。与对照（CK）土壤相比，化肥加涝（CFWL）处理土壤中真菌种类的丰度增加了40.5%，而有机肥加涝（OFWL）处理的土壤中细菌物种丰度增加了10.7％。此外，OFWL 处理的土壤中节杆菌、地杆菌和芽孢杆菌的相对丰度高于 CFWL 处理的土壤（p < 0.01）。在连续单一栽培条件下将 CFWL 应用于温室土壤会产生以真菌为主的土壤，而单独使用 OFWL 会产生以细菌为主的土壤。