The control of mosquito populations using insecticides is increasingly threatened by the spread of resistance mechanisms. Dieldrin resistance, conferred by point mutations in the Rdl gene encoding the γ-aminobutyric acid receptor, has been reported at high prevalence in mosquito populations in response to selective pressures. In this study, we monitored spatio-temporal dynamics of the resistance-conferring RdlR allele in Aedes (Stegomyia) albopictus (Skuse, 1895) and Culex (Culex) quinquefasciatus (Say, 1823) populations from Reunion Island. Specimens of both mosquito species were sampled over a 12-month period in three cities and in sites located at lower (<61 m) and higher (between 503 and 564 m) altitudes. Mosquitoes were genotyped using a molecular test detecting the alanine to serine substitution (A302S) in the Rdl gene. Overall, the RdlR frequencies were higher in Cx. quinquefasciatus than Ae. albopictus. For both mosquito species, the RdlR frequencies were significantly influenced by location and altitude with higher RdlR frequencies in the most urbanized areas and at lower altitudes. This study highlights environmental factors that influence the dynamics of insecticide resistance genes, which is critical for the management of insecticide resistance and the implementation of alternative and efficient vector control strategies.

中文翻译：

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留尼汪岛白纹伊蚊和库蚊种群中狄氏剂抗性基因的时空动态。

使用杀虫剂控制蚊子种群越来越受到抗性机制传播的威胁。狄氏剂抗性由编码 γ-氨基丁酸受体的 Rdl 基因中的点突变赋予，据报道，在蚊子种群中响应选择压力的情况非常普遍。在这项研究中，我们监测了留尼汪岛白纹伊蚊 (Stegomyia) (Skuse, 1895) 和库蚊 (Culex) quinquefasciatus (Say, 1823) 种群中赋予抗性的 RdlR 等位基因的时空动态。在 12 个月的时间里，在三个城市以及海拔较低（<61 m）和较高（503 至 564 m）的地点对两种蚊子的标本进行了采样。使用检测 Rdl 基因中丙氨酸到丝氨酸取代 (A302S) 的分子测试对蚊子进行基因分型。全面的，Cx 中的 RdlR 频率较高。quinquefasciatus 比 Ae。白纹病。对于这两种蚊子，RdlR 频率受到位置和海拔高度的显着影响，在城市化程度最高的地区和低海拔地区，RdlR 频率较高。这项研究强调了影响杀虫剂抗性基因动态的环境因素，这对于管理杀虫剂抗性和实施替代和有效的媒介控制策略至关重要。