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Green Multiplex Chromatographic Determination of Nine Penicillin Antibiotics Residues in Industrial Air Dust and Wastewater Environmental Samples.
Journal of Chromatographic Science ( IF 1.3 ) Pub Date : 2023-08-19 , DOI: 10.1093/chromsci/bmac036
Eman I El-Kimary 1 , Mohammed A Korany 1 , Ahmed E Issa 2 , Mohamed G Basuny 3
Affiliation  

Determination of penicillin residues in different industrial effluents including wastewater and air samples is important to prevent exposure to residual amounts of penicillin and the development of antibiotic resistance. A green high performance liquid chromatography (HPLC) method coupled with diode array detection has been developed and validated for multiplex determination of nine penicillin antibiotics in the industrial air dust and wastewater environmental samples of penicillin facility in addition to the monitoring of facility surface cleaning. Separation was performed on C18 column with gradient elution of methanol and phosphate buffer (pH 4) at a flow rate of 1.5 mL min-1 and ultra violet (UV) detection at 220 nm. Low limits of detection were achieved (0.1-0.3 μg mL-1) indicating good sensitivity of the proposed. The method was applied for ensuring the efficiency of cleaning validation after worst-case selection. Recovery studies of the studied penicillins from fortified stainless steel and polycarbonate surfaces and swabs were between 91.91 and 100.22% with relative standard deviation 0.11-1.79%. The presence of any of the studied penicillins in wastewater samples from penicillin plant drainage was checked. Also, total air dust concentration (mg m-3) and % of penicillin active material residues in air dust were calculated from the area of the exposed group in suspension, tablet and vial production lines. The proposed method can be recommended for routine analysis of air and wastewater environmental samples for the detection of penicillin antibiotics at low levels as well as monitoring of facility surface cleaning with high accuracy and precision.

中文翻译:

绿色多重色谱法测定工业空气灰尘和废水环境样品中的九种青霉素抗生素残留。

测定不同工业废水(包括废水和空气样品)中的青霉素残留对于防止接触残留青霉素和产生抗生素耐药性非常重要。开发并验证了一种与二极管阵列检测相结合的绿色高效液相色谱(HPLC)方法,除了监测设施表面清洁外,还可以多重测定青霉素设施工业空气灰尘和废水环境样品中的九种青霉素抗生素。在 C18 柱上进行分离,采用甲醇和磷酸盐缓冲液 (pH 4) 进行梯度洗脱,流速为 1.5 mL min-1,并在 220 nm 处进行紫外 (UV) 检测。实现了低检测限(0.1-0.3 μg mL-1),表明所提出的方法具有良好的灵敏度。该方法用于确保最坏情况选择后清洁验证的效率。所研究的青霉素从强化不锈钢和聚碳酸酯表面以及拭子中的回收率在 91.91% 至 100.22% 之间,相对标准偏差为 0.11-1.79%。检查青霉素工厂排水废水样品中是否存在任何所研究的青霉素。此外,根据悬浮液、片剂和小瓶生产线中暴露组的面积计算空气粉尘中总空气粉尘浓度(mg m-3)和空气粉尘中青霉素活性物质残留百分比。该方法可推荐用于空气和废水环境样品的常规分析,以检测低含量的青霉素抗生素,以及高精度和高精度地监测设施表面清洁。
更新日期:2022-05-06
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