H₂O₂ and glutathione (GSH) are critical redox molecules in the organism. Abnormal levels of cellular H₂O₂ and GSH are closely related to some diseases. Thus, it is imperative to detect H₂O₂ and GSH efficiently. In this work, FeNC single atom nanozymes (SANs) with both peroxidase and oxidase-mimicking activities were successfully prepared with the help of formamide condensation by one-step hydrothermal method. The FeNC SANs show excellent peroxidase-like activity, which possess a higher affinity for H₂O₂ and 3,3′,5,5′-tetramethylbenzidine (TMB) than horseradish peroxidase (HRP). Then, based on the chromogenic reaction of TMB, a colorimetric biosensor to detect H₂O₂ and GSH was developed. This biosensor has the linear ranges of 10–600 μmol/L for H₂O₂ with a low detection limit of 4.360 μmol/L and 100–400 μmol/L for GSH with a low detection limit of 78.33 μmol/L. Besides, this colorimetric biosensor exhibited a good recovery of H₂O₂ and GSH in diluted human serum. Finally, the FeNC SANs were encapsulated into agar gel to self-quantitatively detect GSH by naked eyes. This work provides a non-pyrolytic way to prepare SANs, which broadens the synthetic method of SANs and may promote the development of SANs for biosensor application.

H ₂ O ₂和谷胱甘肽 (GSH) 是生物体内的关键氧化还原分子。_{细胞H 2} O ₂和GSH水平异常与某些疾病密切相关。因此，高效检测H ₂ O ₂和GSH势在必行。在这项工作中，通过一步水热法通过甲酰胺缩合成功制备了具有过氧化物酶和模拟氧化酶活性的Fe N C 单原子纳米酶 (SANs)。Fe N C SANs表现出优异的过氧化物酶样活性，对H ₂ O ₂具有更高的亲和力和 3,3',5,5'-四甲基联苯胺 (TMB) 比辣根过氧化物酶 (HRP)。然后，基于TMB的显色反应，研制了一种检测H ₂ O ₂和GSH的比色生物传感器。该生物传感器对 H ₂ O ₂的线性范围为 10-600 μmol/L，低检测限为 4.360 μmol/L，对于 GSH 的线性范围为 100-400 μmol/L，低检测限为 78.33 μmol/L。此外，该比色生物传感器在稀释的人血清中表现出良好的 H ₂ O _{2和 GSH 回收率。}最后，Fe N将 C SAN 封装在琼脂凝胶中，通过肉眼自定量检测 GSH。该工作提供了一种非热解制备SANs的方法，拓宽了SANs的合成方法，并可能促进SANs在生物传感器应用方面的发展。