There are three races of Fusarium oxysporum f.sp. cubense (Foc) that significantly affect commercial banana plantations. Most banana cultivars are very susceptible to infection by tropical race 4 of Foc (FocR4T); in contrast, some banana varieties (Cavendish group) are resistant to FocR1 and FocR2. Therefore, it is necessary to develop combined management strategies for controlling the imminent arrival of FocR4T in Mexican plantations. This research evaluated, under in vitro conditions, the effect of seven antagonist bacterial strains, six systemic fungicides, and a fourth generation quaternary ammonium salt on the mycelial development of five Foc strains isolated from banana plantations at Puebla, Michoacan and Oaxaca states (Mexico). Paenibacillus sp. (BSP 1.1) resulted in the highest antagonistic effect (46.6% inhibition) towards the five Foc strains, followed by Serratia sp. (AC35) with 26.6% fungal inhibition. Tebuconazole applied in doses of 50, 100 and 500 μg mL⁻¹ of active ingredient inhibited fungal growth by 100%, whereas Carbendazim and Benomyl delivered 99% fungal inhibition. A high dose of fourth generation quaternary ammonium salts (1250 μg mL⁻¹) caused fungal growth inhibition of 100% and 98.5% after 5 and 8 days post inoculation, respectively. Overall, Paenibacillus sp. (BSP 1.1) and Serratia sp. (AC35) were the most effective bacterial strains; moreover, the application of certain doses of systemic fungicides such as Tebuconazol (50 μg mL⁻¹), Carbendazim and Benomyl (>500 μg mL⁻¹), and ammonium quaternary salts at doses of 1250 μg mL⁻¹ may be considered as alternatives for preventing dissemination, or for using them as part of FocR4T control and sanitary programs.

Fusarium oxysporum f.sp共​​有三个种族。cubense (Foc) 显着影响商业香蕉种植园。大多数香蕉品种非常容易受到热带第 4 种 Foc (FocR4T) 的感染；相比之下，一些香蕉品种（卡文迪什组）对 FocR1 和 FocR2 具有抗性。因此，有必要制定联合管理策略来控制 FocR4T 即将抵达墨西哥种植园。本研究在体外条件下评估了七种拮抗细菌菌株、六种内吸性杀菌剂和第四代季铵盐对从普埃布拉州、米却肯州和瓦哈卡州（墨西哥）香蕉种植园分离的五种 Foc 菌株的菌丝体发育的影响。 . 类芽孢杆菌sp。(BSP 1.1) 对五种 Foc 菌株产生最高的拮抗作用（46.6% 抑制），其次是沙雷氏菌。(AC35) 具有 26.6% 的真菌抑制作用。戊唑醇以 50、100 和 500 μg mL ^-1的剂量施用活性成分可抑制 100% 的真菌生长，而多菌灵和苯菌灵则提供 99% 的真菌抑制作用。接种后5天和8天后，高剂量的第四代季铵盐(1250 μg mL ^{-1 )分别引起100%和98.5%的真菌生长抑制。}总体而言，类芽孢杆菌属。(BSP 1.1) 和沙雷氏菌sp。(AC35) 是最有效的菌株；此外，可以考虑使用特定剂量的内吸性杀菌剂，例如戊唑醇 (50 μg mL ^-1 )、多菌灵和苯菌灵 (>500 μg mL ^{-1 )，以及 1250 μg mL -1}剂量的季铵盐。用于防止传播，或将它们用作 FocR4T 控制和卫生计划的一部分。