Abstract

In this study, R-phycoerythrin (R-PE) was isolated and characterized from Porphyra yezoensis by three-phase partitioning (TPP) method. The effects of temperature, time, pH, salt saturation, and volume ratio on the purity and recovery rate of R-PE were studied. The optimum extraction conditions were determined as follows: salt saturation of 70%, temperature of 25 °C, time of 45 min, pH of 7.0, and volume ratio of 1:1. Under the optimal extraction conditions, the purity of R-PE was 3.90. The results of SDS-PAGE showed that R-PE has three bands at 23 kDa, 22 kDa, and 18 kDa, corresponding to its α, β, γ subunits. The structure and optical activity of R-PE did not change before and after purification based on ultraviolet, infrared, and fluorescence spectra. In addition, the purity and recovery rate of R-PE extracted by tert-butanol were evaluated. The results showed that the extraction performance of tert-butanol for R-PE remained unchanged in three recoveries. These show that TPP is an efficient, green, and recyclable extraction technology.

摘要

在本研究中，从条斑紫菜中分离并表征了 R-藻红蛋白 (R-PE)通过三相分配（TPP）方法。研究了温度、时间、pH、盐饱和度和体积比对R-PE纯度和回收率的影响。确定最佳提取条件为：盐饱和度70%、温度25 °C、时间45 min、pH 7.0、体积比1:1。在最佳提取条件下，R-PE的纯度为3.90。SDS-PAGE结果显示R-PE在23 kDa、22 kDa和18 kDa处有3条带，分别对应其α、β、γ亚基。根据紫外、红外和荧光光谱，R-PE 的结构和光学活性在纯化前后没有变化。此外，评价了叔丁醇提取的R-PE的纯度和回收率。结果表明，叔丁醇对R-PE的萃取性能在三次回收中均保持不变。这些表明TPP是一种高效、绿色、可回收的提取技术。