Compared with the substantial amount of genomics data generated in recent years, phenotypic data availability for crops is largely restricted in spectrum and quantity. High-throughput phenotyping (HTP) platforms have been developed to overcome this limitation, not only by enhancing the speed and accuracy of phenotyping but also by expanding the range of evaluable traits through integration of various imaging systems. Three-dimensional (3D) imaging enables non-destructive estimation of plant volume and structure. Hyperspectral imaging (HSI) generates reflectance data for a broad range of wavelengths that are associated with diverse physiological traits. We performed a pilot study to apply 3D and HSI for investigation of heterosis and cytoplasmic effects that have been evaluated previously based on manually analyzable traits. The plant volumes estimated from 3D images by a customized pipeline quantitatively showed heterosis in three cross combinations between genetically distant pepper (Capsicum annuum) accessions. The normalized difference vegetation index (NDVI) derived from HSI reflectance data also showed differences between parental and F₁ lines. The cytoplasmic effects investigated by comparison of F₁ lines derived from reciprocal crosses was subtle and thus was not detected in plant volume or NDVI values. However, the difference between F₁ lines was detected in a subset of cross combinations at a specific developmental stage based on reflectance for wavelength ranges selected as the most relevant. The results showed the potential utility of HSI data recorded throughout the plant life-span for analysis of cytoplasmic effects. The present study demonstrated the applicability of a HTP platform integrated with 3D and HSI systems for evaluation of combining ability in crops.

与近年来产生的大量基因组学数据相比，作物表型数据的可用性在很大程度上受到光谱和数量的限制。已经开发了高通量表型 (HTP) 平台来克服这一限制，不仅通过提高表型分析的速度和准确性，而且通过集成各种成像系统来扩大可评估性状的范围。三维 (3D) 成像能够对植物体积和结构进行无损估计。高光谱成像 (HSI) 生成与多种生理特征相关的广泛波长的反射率数据。我们进行了一项试点研究，以应用 3D 和 HSI 来研究先前基于手动可分析性状评估的杂种优势和细胞质效应。Capsicum annuum ) 种质。从 HSI 反射率数据得出的归一化差异植被指数 (NDVI) 也显示了亲本系和 F ₁系之间的差异。通过比较来自互交的 F _{1系所研究的细胞质效应是微妙的，因此在植物体积或 NDVI 值中未检测到。但是，F 1}之间的差异根据选择为最相关的波长范围的反射率，在特定发育阶段的交叉组合子集中检测到线。结果表明，在整个植物生命周期中记录的 HSI 数据在分析细胞质效应方面具有潜在的用途。本研究证明了与 3D 和 HSI 系统集成的 HTP 平台在评估作物配合力方面的适用性。