There have been a few reports on the impacts of arbuscular mycorrhizal fungi (AMF) on the expression of genes associated with the uptake, transport and detoxification of Cd in upland rice, but there is no study on the impacts of AMF on the transcriptome of upland rice grown in Cd-contaminated soil. In the present work, a pot experiment was conducted to study the influences of the AMF-Glomus versiforme (Gv) on the Cd accumulation, photosynthesis, antioxidant enzymes and transcriptome in upland rice grown in soils supplemented with 10 μg Cd g⁻¹ soil. Gv symbiosis evidently promoted the plant growth, P acquisition and photosynthetic characteristics in upland rice. In addition, 229 differentially expressed genes (DEGs) were identified in upland rice root inoculated with Gv, of which 149 DEGs were upregulated and 80 DEGs were downregulated. More importantly, one Cd transporter gene Nramp5 was significantly downregulated, thus reducing Cd absorption, transfer and accumulation in mycorrhizal upland rice. Moreover, four genes in the Gene Ontology (GO) term of cysteine biosynthesis and two metallothionein genes were upregulated, which could have decreased Cd phytotoxicity in upland rice inoculated with Gv. Meanwhile, one peroxidase gene, two genes in the GO term of peroxisome and one gene in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of carotenoid biosynthesis were significantly upregulated, and enhanced the activities of catalase (CAT) and peroxidase (POD) in Gv-inoculated upland rice. Our results of transcriptome analysis initially elucidated the molecular mechanism by which AMF decreased the accumulation and toxicity of Cd in upland rice.

丛枝菌根真菌(AMF)对旱稻Cd吸收、转运和解毒相关基因表达的影响报道较少，但AMF对旱稻转录组的影响尚无研究。在镉污染土壤中种植的水稻。在本工作中，进行了盆栽试验，研究了 AMF- Glomus versiforme (Gv) 对在补充 10 μg Cd g ^-1的土壤中生长的旱稻中 Cd 积累、光合作用、抗氧化酶和转录组的影响。土壤。Gv共生显着促进了旱稻的植株生长、磷的获取和光合特性。此外，在接种Gv的旱稻根系中鉴定出229个差异表达基因（DEGs），其中上调149个，下调80个。更重要的是，一个 Cd 转运蛋白基因Nramp5显着下调，从而减少了菌根旱稻中 Cd 的吸收、转移和积累。此外，半胱氨酸生物合成的基因本体（GO）术语中的四个基因和两个金属硫蛋白基因被上调，这可能降低了接种Gv的旱稻中镉的植物毒性。同时，1个过氧化物酶基因、2个过氧化物酶体GO术语基因和1个京都基因与基因组百科全书（KEGG）类胡萝卜素生物合成途径基因显着上调，并增强了过氧化氢酶（CAT）和过氧化物酶（POD）的活性。在 Gv 接种的旱稻中。我们的转录组分析结果初步阐明了 AMF 降低旱稻 Cd 积累和毒性的分子机制。