This study was carried out in the tissue culture laboratory of the Horticulture Department of the Faculty of Agriculture of Ordu University, Turkey, during the vegetation period of 2019. As plant material, the shoot tips of Vitis labrusca L. (Balikci Siyahi) genotype were used during the active growth period. The explants were cultured in Murashige and Skoog (MS) nutrient medium containing 1 mg/l 6‑benzylaminopurin (BAP) to form shoots after surface sterilization. Growing shoots from explants cultured in BAP media were transferred to MS nutrient medium containing 1 mg/l IBA and five different polyethylene glycol (PEG) doses (0 %, 1.5 %, 3.0 %, 4.5 %, 6.0 %) during rooting. Plant viability (%), shoot length (cm), number of shoot internodes (n), number of leaves in the shoot (n), chlorophyll amount, shoot fresh weight (g), shoot dry weight (g), fresh and dry weight of leaves (g), cell membrane damage rate (%), degree of damage, shoot tolerance rate, leaf turgor weight (g), explant proportional water scope (%), ion flow (%), number of roots (n), root length (cm), and fresh and dry weights of roots (g) were examined at the end of 3 weeks of culture on these shoots. As a result, with increasing PEG doses, it was determined that cell membrane damage rate, damage level, and ion flow increased. Growth and development, wet and dry weights of plant, root number, root length, and wet and dry weights of root decreased.

本研究在土耳其奥尔杜大学农学院园艺系组织培养实验室于 2019 年植被期间进行。作为植物材料，Vitis labrusca  L. (Balikci Siyahi) 基因型的茎尖在活跃的生长期使用。外植体在含有 1 mg/l 6-苄氨基嘌呤 (BAP) 的 Murashige 和 Skoog (MS) 营养培养基中培养，表面灭菌后形成枝条。在生根期间，将在 BAP 培养基中培养的外植体的生长芽转移到含有 1 mg/l IBA 和五种不同聚乙二醇 (PEG) 剂量（0%、1.5%、3.0%、4.5%、6.0%）的 MS 营养培养基中。植物活力 (%)、枝条长度 (cm)、枝条节间数 ( n )、枝条中的叶片数 (n )、叶绿素量、枝条鲜重(g)、枝条干重(g)、叶片鲜干重(g)、细胞膜损伤率(%)、损伤程度、枝条耐受率、叶膨压( g)、外植体比例水分范围(%)、离子流(%)、根数( n )、根长(cm)、根干鲜重(g)在培养3周结束时检测在这些芽上。结果，随着PEG剂量的增加，确定细胞膜损伤率、损伤水平和离子流增加。生长发育、植株干湿重、根数、根长、根干湿重均下降。