当前位置: X-MOL 学术Biomass Bioenergy › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Valorization of agricultural residues using Myceliophthora thermophila as a platform for production of lignocellulolytic enzymes for cellulose saccharification
Biomass & Bioenergy ( IF 6 ) Pub Date : 2022-04-25 , DOI: 10.1016/j.biombioe.2022.106452
Nathália Gonsales da Rosa-Garzon , Hélen Julie Laure , José César Rosa , Hamilton Cabral

The use of lignocellulosic agricultural residues is emerging as a low-cost, sustainable, and eco-friendly approach for the production of microbial enzymes and other chemicals. In this study, the production of enzymes with lignocellulolytic potential and the secretome profile of the thermophilic fungus Myceliophthora thermophila were evaluated in solid-state cultivation on agricultural residues such as rice straw, soy straw, and wheat bran. In addition, the saccharification potential of the extracts was evaluated. The analysis of enzymatic activities (peptidase, lipase, amylase, CMCase, FPase, β-glucosidase, xylanase, pectinase, and laccase) and the 66 proteins identified in the secretome reinforced the hydrolytic and oxidative properties of the enzymatic lignocellulolytic arsenal produced by M. thermophila. The extracellular extract grown on rice straw obtained after 72 h of solid-state bioprocess achieved 46% saccharification of wheat bran substrate. Furthermore, the overall performance of the wheat bran extract (24 h) indicates the relevance of the CMCase, FPase and xylanase activities in saccharification processes. These results highlight the potential of M. thermophila as a platform for the production of enzymes with biotechnological potential, especially for lignocellulose degradation and cellulose saccharification. Solid-state bioprocess allows exploring agro-industrial residues, as a matrix and source of nutrients, for the enzyme production and emphasizes the possibility of adding value to agricultural waste. Moreover, the use of M. thermophila in solid-state bioprocesses can still be widely explored and optimized as a platform to scale up the production of cheapest fungal enzymes.



中文翻译:

使用嗜热毁丝霉作为生产用于纤维素糖化的木质纤维素分解酶的平台对农业残留物进行估价

木质纤维素农业残留物的使用正在成为生产微生物酶和其他化学品的低成本、可持续和环保的方法。在这项研究中,在水稻秸秆、大豆秸秆和麦麸等农业残留物的固态培养中,评估了具有木质纤维素分解潜力的酶的生产和嗜热真菌嗜热毁丝霉的分泌组谱。此外,评价了提取物的糖化潜力。对酶活性(肽酶、脂肪酶、淀粉酶、CMCase、FPase、β-葡糖苷酶、木聚糖酶、果胶酶和漆酶)和分泌组中鉴定的 66 种蛋白质的分析增强了由嗜热菌。在经过 72 小时的固态生物处理后,在稻草上生长的细胞外提取物实现了 46% 的麦麸底物糖化。此外,麦麸提取物(24 小时)的整体性能表明 CMCase、FPase 和木聚糖酶活性在糖化过程中的相关性。这些结果突出了嗜热分枝杆菌作为生产具有生物技术潜力的酶的平台的潜力,特别是对于木质纤维素降解和纤维素糖化。固态生物工艺允许探索农业工业残余物,作为基质和营养来源,用于酶的生产,并强调为农业废物增加价值的可能性。此外,使用M. thermophila在固态生物过程中,仍然可以广泛探索和优化,作为扩大生产最便宜的真菌酶的平台。

更新日期:2022-04-27
down
wechat
bug