Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. These advantages come with additional responsibilities and challenges given that many assays and platforms are not provided to laboratories as a single kit or device. The skills, staff, and assays used in LC-MS/MS are internally developed by the laboratory, with relatively few exceptions. Hence, a laboratory that deploys LC-MS/MS assays must be conscientious of the practices and procedures adopted to overcome the challenges associated with the technology. This review discusses the post-development landscape of LC-MS/MS assays, including validation, quality assurance, operations, and troubleshooting. The content knowledge of LC-MS/MS users is quite broad and deep and spans multiple scientific fields, including biology, clinical chemistry, chromatography, engineering, and MS. However, there are no formal academic programs or specific literature to train laboratory staff on the fundamentals of LC-MS/MS beyond the reports on method development. Therefore, depending on their experience level, some readers may be familiar with aspects of the laboratory practices described herein, while others may be not. This review endeavors to assemble aspects of LC-MS/MS operations in the clinical laboratory to provide a framework for the thoughtful development and execution of LC-MS/MS applications.

中文翻译：

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液相色谱-串联质谱在临床实验室中的应用回顾：第二部分-操作。

液相色谱-串联质谱 (LC-MS/MS) 越来越多地用于临床实验室，因为它在特异性和灵敏度方面优于其他分析技术。鉴于许多检测和平台不是作为单一试剂盒或设备提供给实验室的，这些优势伴随着额外的责任和挑战。LC-MS/MS 中使用的技能、人员和分析由实验室内部开发，相对较少的例外。因此，部署 LC-MS/MS 分析的实验室必须认真对待为克服与该技术相关的挑战而采用的实践和程序。本综述讨论了 LC-MS/MS 分析的后开发情况，包括验证、质量保证、操作和故障排除。LC-MS/MS用户的内容知识相当广泛和深入，跨越多个科学领域，包括生物学、临床化学、色谱、工程和MS。然而，除了方法开发报告之外，没有正式的学术课程或特定文献来培训实验室工作人员了解 LC-MS/MS 的基础知识。因此，根据他们的经验水平，一些读者可能熟悉本文描述的实验室实践的各个方面，而其他人可能不熟悉。本综述力图整合临床实验室中 LC-MS/MS 操作的各个方面，为深入开发和执行 LC-MS/MS 应用程序提供一个框架。除了方法开发报告之外，没有正式的学术课程或特定文献来培训实验室工作人员了解 LC-MS/MS 的基础知识。因此，根据他们的经验水平，一些读者可能熟悉本文描述的实验室实践的各个方面，而其他人可能不熟悉。本综述力图整合临床实验室中 LC-MS/MS 操作的各个方面，为深入开发和执行 LC-MS/MS 应用程序提供一个框架。除了方法开发报告之外，没有正式的学术课程或特定文献来培训实验室工作人员了解 LC-MS/MS 的基础知识。因此，根据他们的经验水平，一些读者可能熟悉本文描述的实验室实践的各个方面，而其他人可能不熟悉。本综述力图整合临床实验室中 LC-MS/MS 操作的各个方面，为深入开发和执行 LC-MS/MS 应用程序提供一个框架。