The estrogen-related receptors (ERRs), a group of nuclear receptors, are the first orphan receptor to be identified. In this study, six errs (errα, errβ, errγ, errγA, errγB, errδ) were first cloned from the gonads of mandarin fish (Siniperca chuatsi). Gene cloning results showed the open reading frames (ORF) of both errα, errβ, errγ, errγA, errγB, errδ were 1302 bp, 1434 bp, 1323 bp, 1566 bp and 1308 bp, coding a 432, 476, 439, 520, 434 and 337 amino acid (AA) peptide, respectively. Tissue distribution analysis showed that errα was relatively highly expressed in heart and muscle both in male and female and errγA was highly expressed in brain, stomach and muscle while the errγB was extremely highly expressed in heart and liver both in male and female. The errβ expressed relatively highly in medulla oblongata and gonad of male and female and kidney of male, while errγ expressed relatively highly in stomach of male and female. Gonad expression pattern revealed that errα, errγ, errγA and errγB were all detected from 5 to 180 day after hatching (dah) in both males and females, and errδ began to express after 60 dah. Moreover, errγ, errγA and errγB were highly expressed in 5–10 dah while errα and errδ were mainly expressed in 60–180 dah. Furthermore, luciferase assay showed that errγ could response to estrogen stimulation by modulating estrogen-signaling pathway in mandarin fish.

雌激素相关受体 (ERR) 是一组核受体，是第一个被鉴定的孤儿受体。本研究首先从鳜鱼（Siniperca chuatsi ）的性腺中克隆出6个err（ errα、errβ、errγ、errγA、errγB、errδ）。基因克隆结果显示，errα、errβ、errγ、errγA、errγB、errδ的开放阅读框（ORF）分别为1302 bp、1434 bp、1323 bp、1566 bp和1308 bp，分别编码432、476、439、520、分别为 434 和 337 个氨基酸 (AA) 肽。组织分布分析表明，errα在男性和女性的心脏和肌肉中均相对高表达，errγA在脑、胃和肌肉中高表达，errγB在男性和女性的心脏和肝脏中都高度表达。errβ在男性和女性的延髓、性腺和男性的肾脏中表达相对较高，而errγ在男性和女性的胃中表达相对较高。性腺表达模式显示，errα、errγ、errγA和errγB均在雄性和雌性孵化后 5 至 180 天（dah）检测到，errδ在 60 dah 后开始表达。此外，errγ、errγA和errγB在 5-10 dah 中高表达，而errα和errδ主要在 60-180 dah 中表达。此外，荧光素酶测定表明，errγ可通过调节鳜鱼的雌激素信号通路对雌激素刺激作出反应。