In Kuwait, some untreated sewage is discharged into the sea which poses health risks. Therefore, we determined the virulence traits and the phylogenetic groups of E. coli cultured from raw sewage. Sewage was collected once every month for 12 months with culturing of a total of 140 E. coli isolates. E. coli was typed by the methods of Clermont. The five pathotypes of diarrheagenic E. coli (DEC), and extra-intestinal pathogenic E. coli (ExPEC) were detected by specific PCR assays. Four virulence genes which correlate with pathogenicity in animal models, were used for the first time for detection of ExPEC—vat (vacuolating auto-transporter toxin), fyuA (yersiniabactin receptor), chuA (heme-binding protein), and yfcV (major subunit of putative chaperon-usher fimbria). Most E. coli belonged to phylogenetic groups A (65[45%]) and B1 (34[24.3%]). Three (2.1%) isolates were DEC, while 14 (10%) isolates were ExPEC mostly in group B2 (57.1%). A relatively high prevalence of ExPEC in sewage has public health implications.

中文翻译：

---

科威特原污水培养的大肠杆菌的毒力和系统发育群

在科威特，一些未经处理的污水排入大海，对健康构成威胁。因此，我们确定了从原污水中培养的大肠杆菌的毒力性状和系统发育组。每月收集一次污水，持续 12 个月，共培养 140 株大肠杆菌。大肠杆菌通过克莱蒙的方法进行分型。通过特异性 PCR 分析检测了腹泻性大肠杆菌 (DEC) 和肠外致病性大肠杆菌 (ExPEC) 的五种致病型。动物模型中与致病性相关的四种毒力基因首次用于检测 ExPEC——vat（空泡化自转运体毒素）、fyuA（耶尔森菌素受体）、chuA（血红素结合蛋白）和 yfcV（主要亚基）假定的伴侣-引座菌菌毛）。大多数大肠杆菌属于系统发育组 A (65[45%]) 和 B1 (34[24.3%])。三（2。1%) 分离株是 DEC，而 14 个 (10%) 分离株是 ExPEC，主要在 B2 组 (57.1%)。污水中相对较高的 ExPEC 发生率具有公共卫生影响。