当前位置: X-MOL 学术ISME J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Photophysiological response of Symbiodiniaceae single cells to temperature stress
The ISME Journal ( IF 11.0 ) Pub Date : 2022-04-26 , DOI: 10.1038/s41396-022-01243-6
Linhong Xiao 1 , Sofia Johansson 2 , Saskia Rughöft 1 , Fabien Burki 1 , Miguel Mendez Sandin 1 , Maria Tenje 2 , Lars Behrendt 1
Affiliation  

Photosynthetic dinoflagellates in the family Symbiodiniaceae engage in symbiosis with scleractinian corals. As coral ‘bleaching’ is partly governed by the thermal sensitivity of different Symbiodiniaceae lineages, numerous studies have investigated their temperature sensitivity. However, the systematic identification of single-cells with increased temperature resistance among these dinoflagellates has remained inaccessible, mostly due to a lack of technologies operating at the microscale. Here, we employed a unique combination of microfluidics, miniaturized temperature control, and chlorophyll fluorometry to characterize the single-cell heterogeneity among five representative species within the Symbiodiniaceae family under temperature stress. We monitored single-cell maximum quantum yields (Fv/Fm) of photosystem (PS) II under increasing temperature stress (22‒39 °C, + 1 °C every 15 min), and detected a significant Fv/Fm reduction at lineage-specific temperatures ranging from 28 °C to 34 °C alongside a 40- to 180- fold increase in intraspecific heterogeneity under elevated temperatures (>31 °C). We discovered that the initial Fv/Fm of a cell could predict the same cell’s ability to perform PSII photochemistry under moderate temperature stress (<32 °C), suggesting its use as a proxy for measuring the thermal sensitivity among Symbiodiniaceae. In combination, our study highlights the heterogeneous thermal sensitivity among photosynthetic Symbiodiniaceae and adds critical resolution to our understanding of temperature-induced coral bleaching.



中文翻译:

共生藻科单细胞对温度胁迫的光生理反应

共生藻科中的光合甲藻与巩膜珊瑚共生。由于珊瑚“白化”部分受不同共生藻科谱系的热敏感性控制,因此许多研究调查了它们的温度敏感性。然而,在这些甲藻中系统地识别具有更高耐温性的单细胞仍然无法获得,这主要是由于缺乏在微尺度上运行的技术。在这里,我们采用微流体、小型化温度控制和叶绿素荧光测定的独特组合来表征温度胁迫下共生藻科中五个代表性物种的单细胞异质性。我们监测了单细胞最大量子产率 ( F v /F m ) 光系统 ( PS ) II 在不断升高的温度胁迫下(22-39 °C,每 15 分钟 + 1 °C),并检测到28 ° C至 28 °C 至34 °C 以及高温 (>31 °C) 下种内异质性增加 40 至 180 倍。我们发现最初的F v / F m细胞的 可以预测同一细胞在中等温度胁迫 (<32 °C) 下进行 PSII 光化学的能力,表明它可以用作测量共生藻科热敏感性的代理。结合起来,我们的研究强调了光合作用共生藻科之间的异质热敏感性,并为我们对温度引起的珊瑚白化的理解增加了关键分辨率。

更新日期:2022-04-26
down
wechat
bug