There are common significant character differences between genders in many fish. The sex-specific genetic markers are important tools for research and industry. Golden pompano (Trachinotus blochii) is a commercial mass-produced species with sex growth differences. Our study includes the whole genome re-sequencing data of T. blochii from four populations. A total of 2,183,486 high-quality clean reads were obtained for subsequent information analysis. Association analysis of single nucleotide polymorphism markers with sex was carried out, the genome-wide association studyGWAS was carried out using the simple linear regression model (Q), and a high-scored site loci in LG20 exhibiting significant association with sex was found. A heterozygous mutation was observed in this site in female fish (G/A), and a homozygous mutation in males (A/A). Within the 50kb annotation interval of this variant site, hsd17b1 was found to be linked to sex-related pathways significantly. Based on the site, we developed a simple and efficient polymerase chain reaction-based sex-specific molecular marker for identifying the T. blochii genetics sex. It will benefit the sex-controlled breeding and sex development study of the golden pompano.

中文翻译：

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金鲳 (Trachinotus blochii) 性别特异性分子标记的开发与验证

许多鱼的性别之间存在共同的显着性状差异。性别特异性遗传标记是研究和工业的重要工具。金鲳鱼（Trachinotus blochii）是一种具有性别生长差异的商业化大量生产物种。我们的研究包括T的全基因组重测序数据。布洛基来自四个人群。共获得 2,183,486 条高质量的干净 reads 用于后续信息分析。进行单核苷酸多态性标记与性别的关联分析，使用简单线性回归模型（Q）进行全基因组关联研究GWAS，发现LG20中与性别显着关联的高分位点位点。在雌性鱼的该位点观察到杂合突变（G/A），在雄性鱼中观察到纯合突变（A/A）。在该变异位点的 50kb 注释区间内，发现hsd17b1与性相关通路显着相关。基于该站点，我们开发了一种简单有效的基于聚合酶链反应的性别特异性分子标记，用于鉴定T。blochii遗传学性别。有利于金鲳的性控繁育和性发育研究。