The fungal pathogen Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus cultivation and affected mushrooms significantly reduce the yield and revenue for mushroom growers. Biocontrol agents may represent an alternative and more environmentally friendly treatment option to help control dry bubble on mushroom farms. Serenade ® is a commercially available biocontrol product used for disease treatment in plant crops. In this work, the in vitro response of L. fungicola to the bacterial strain active in Serenade, Bacillus velezensis (QST 713) and a newly isolated B. velezensis strain (Kos) was assessed. B. velezensis (QST713 and Kos) both produced zones of inhibition on plate cultures of L. fungicola, reduced the mycelium growth in liquid cultures and damaged the morphology and structure of L. fungicola hyphae. The proteomic response of the pathogen against these biocontrol strains was also investigated. Proteins involved in growth and translation such as 60S ribosomal protein L21-A (−32-fold) and 40S ribosomal protein S30 (−17-fold) were reduced in abundance in B. velezensis QST 713 treated samples, while proteins involved in a stress response were increased (norsolorinic acid reductase B (47-fold), isocitrate lyase (11-fold) and isovaleryl-CoA dehydrogenase (8-fold). L. fungicola was found to have a similar proteomic response when exposed to B. velezensis (Kos). This work provides information on the response of L. fungicola to B. velezensis (QST 713) and indicates the potential of B. velezensis Kos as a novel biocontrol agent.

真菌病原体Lecanicillium funccola在双孢蘑菇栽培中引起干泡病，并且受影响的蘑菇显着降低了蘑菇种植者的产量和收入。生物防治剂可能代表一种替代且更环保的处理选择，以帮助控制蘑菇农场的干泡。Serenade ® 是一种市售的生物防治产品，用于植物作物的疾病治疗。在这项工作中，评估了L.fungicola对小夜曲、Bacillus velezensis (QST 713) 和新分离的B. velezensis菌株 (Kos) 中的活性菌株的体外反应。B. velezensis( QST713和 Kos) 均在平板培养物上产生抑制区，减少液体培养物中的菌丝体生长，并破坏了真菌菌丝的形态和结构。还研究了病原体对这些生物防治菌株的蛋白质组学反应。参与生长和翻译的蛋白质，如 60S 核糖体蛋白 L21-A（-32 倍）和 40S 核糖体蛋白 S30（-17 倍）在B. velezensis QST 713 处理的样品中的丰度减少，而参与压力的蛋白质反应增加（降氯酸还原酶 B（47 倍）、异柠檬酸裂解酶（11 倍）和异戊酰辅酶 A 脱氢酶（8 倍）。发现L. funcola在暴露于B. velezensis (科斯)。这项工作提供了有关L.fungicola对B. velezensis (QST 713) 反应的信息，并表明了B. velezensis Kos 作为新型生物防治剂的潜力。