This study aimed to compare the phenotypic and genotypic characteristics, and pathogenicity of Edwardsiella ictaluri derived from several catfish farming in Indonesia. The isolated bacteria were from West Java, East Java, South Sumatra, Jambi, Lampung, Riau and South Kalimantan. The phenotypic analysis based on conventional biochemical characteristics, API 20E and enzyme activity by APIzym showed that the E. ictaluri from various locations in Indonesia had the different growth patterns, ability to hydrolyze sugar (saccharose and amylase), hydrolyzed gelatin, lysed blood agar media and leucine and valine arylamidase enzyme activity. The sensitivity antibiotic testing showed most of the tested isolates were multi-drug resistance to five antimicrobial classes (macrolides, lincosamides, rifamycin, penicillins and quinolones). The phylogenetic analysis with sequencing 16S rRNA had 99.56%–100% similarity to E. ictaluri. The genotypic analysis of the similarity strains with Genetic Fingerprinting Repetitive sequence-mediated PCR (Rep-PCR) denoted different genotype profiles of each primer ERIC 1 ERIC II, BOX and GTG₅. The protein profiles were analysed using the SDS-PAGE method. The whole-cell isolates of E. ictaluri coded PJbH, P and PBm1G had outer membrane protein (OMP) and riboflavin biosynthesis protein (RibD). The pathogenicity test led to high mortality for 97.78%–100% in the whole-cell treatment, while the low mortality was 6.67% in the ECP treatment. These results concluded that E. ictaluri isolates from various farming in Indonesia had different phenotypic and genotypic variations and protein components of E. ictaluri cells proved to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.

中文翻译：

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引起鲶鱼肠道败血症的印度尼西亚当地分离株Edwardsiella ictaluri的表型、基因型和致病性比较

本研究旨在比较来自印度尼西亚几种鲶鱼养殖场的Edwardsiella ictaluri的表型和基因型特征以及致病性。分离出的细菌来自西爪哇、东爪哇、南苏门答腊、占碑、楠榜、廖内和南加里曼丹。APIzym 基于常规生化特征、API 20E 和酶活性的表型分析表明，E . 伊塔鲁里来自印度尼西亚不同地点的生长模式、水解糖（蔗糖和淀粉酶）、水解明胶、裂解血琼脂培养基以及亮氨酸和缬氨酸芳基酰胺酶活性的能力不同。敏感性抗生素测试显示，大多数被测试的分离株对五种抗菌药物（大环内酯类、林可酰胺类、利福霉素、青霉素和喹诺酮类）具有多重耐药性。用测序 16S rRNA 进行的系统发育分析与E具有 99.56%–100% 的相似性。伊塔鲁里。用遗传指纹重复序列介导的 PCR (Rep-PCR) 对相似菌株的基因型分析表示每个引物 ERIC 1 ERIC II、BOX 和 GTG _{5的不同基因型谱}. 使用 SDS-PAGE 方法分析蛋白质谱。E的全细胞分离物。ictaluri编码的 PJbH、P 和 PBm1G 具有外膜蛋白 (OMP) 和核黄素生物合成蛋白 (RibD)。致病性试验导致全细胞治疗的高死亡率为97.78%~100%，而ECP治疗的低死亡率为6.67%。这些结果得出结论，E。来自印度尼西亚各种农业的ictaluri分离物具有不同的表型和基因型变异以及E的蛋白质成分。ictaluri细胞被证明具有毒性，并在大肠杆菌的发病机制中发挥作用。鲶鱼的ictaluri感染。