The present research comes up with a novel DNA-loaded poly-L-lysine (PLL)/hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of –24 ± 3 mV, with an average size of 138 ± 6 nm, in an ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore, the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimised conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.

中文翻译：

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聚-L-赖氨酸/透明质酸纳米载体作为基因传递的新型纳米系统

本研究提出了一种用于基因递送应用的新型载有 DNA 的聚 L-赖氨酸 (PLL)/透明质酸 (HA) 纳米载体（载有 DNA 的 PLL/HA NCs），作为将基因递送到不同细胞中的有希望的候选者。采用一种直接的方法通过 HA 掩蔽载有 DNA 的 PLL 分子来制备这种纳米系统。傅里叶变换红外 (FTIR) 光谱、动态光散射 (DLS)、场发射扫描电子显微镜 (FE-SEM) 和透射电子显微镜 (TEM) 用于分析分子的相互作用以及物理化学性质NC。NCs 显示负电荷为 –24 ± 3 mV，平均尺寸为 138 ± 6 nm，呈椭圆形，表面光滑。通过 DNA 吸光度测量的 DNA 加载效率 (LE) 约为 95%。MTT 测定表明，开发的 NCs 对细胞无毒。此外，通过流式细胞术方法评估了人胚胎肾 (HEK)-293T 细胞对载有 DNA 的 PLL/HA NCs 的摄取，并证明了将基因转移到 HEK-293T 细胞的潜在细胞摄取率超过 90%在优化的条件下。因此，载有 DNA 的 PLL/HA NC 是开发用于基因传递应用的纳米系统的有效策略。