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Genetic diversity and relationship analyses of mango (Mangifera indica L.) germplasm resources with ISSR, SRAP, CBDP and CEAP markers
Scientia Horticulturae ( IF 4.3 ) Pub Date : 2022-04-20 , DOI: 10.1016/j.scienta.2022.111146
Mei-yan Chen 1 , Xin-hua He 1 , Yi-li Zhang 1 , Ting-ting Lu 1 , Wei-qiao-chu He 1 , Ji-hong Yang 1 , Xing Huang 1 , Jia-wei Zhu 1 , Hai-xia Yu 1 , Cong Luo 1
Affiliation  

Forty-eight mango accessions were compared for genetic diversity and relationships based on four molecular marker assays, namely, inter-simple sequence repeat (ISSR), sequence-related amplified polymorphism (SRAP), CAAT-box derived polymorphism (CBDP), and cis-element amplified polymorphism (CEAP). Comparative analyses of the marker assays based on Nei's genetic diversity (H), Shannon's information index (I), polymorphism information content (PIC), the marker index (MI), and resolving power (Rp) revealed that (i) the CEAP markers provided the most information according to the PIC, MI and Rp values; (ii) the CBDP and ISSR markers exhibited valuable information according to the H and I values. The correlation values (r) of the Mantel test ranged from 0.334 (ISSR and CEAP) to 0.819 (CEAP and the combination of the four marker assays). The unweighted pair group method with arithmetic mean (UPGMA) cluster results showed that a single-marker assay can accurately reflect the genetic relationships of some mango samples but cannot accurately reflect the genetic relationships of all mango samples and that when multiple markers are used to evaluate the genetic relationships of mango germplasm resources, the results are more accurate than those of a single-marker assay. The combined cluster analysis of the molecular marker types shows higher discrimination according to genetic relationships. Nine mango accessions were suggested to be likely parental or sister lines based on the combination of the ISSR, SRAP, CBDP, and CEAP marker assays.



中文翻译:

芒果(Mangifera indica L.)种质资源的ISSR、SRAP、CBDP和CEAP标记遗传多样性及关系分析

基于四种分子标记分析,即简单序列重复间 (ISSR)、序列相关扩增多态性 (SRAP)、CAAT-box 衍生多态性 (CBDP) 和顺式,比较了 48 个芒果种质的遗传多样性和关系-元素扩增多态性(CEAP)。基于 Nei 的遗传多样性 (H)、香农信息指数 (I)、多态性信息含量 (PIC)、标记指数 (MI) 和分辨能力 (Rp) 的标记测定的比较分析表明 (i) CEAP 标记根据 PIC、MI 和 Rp 值提供的信息最多;(ii) CBDP 和 ISSR 标记根据 H 和 I 值显示有价值的信息。Mantel 检验的相关值 (r) 范围从 0.334(ISSR 和 CEAP)到 0.819(CEAP 和四种标志物测定的组合)。具有算术平均值的未加权配对组法(UPGMA)聚类结果表明,单标记测定可以准确反映部分芒果样品的遗传关系,但不能准确反映所有芒果样品的遗传关系,当使用多个标记进行评估时芒果种质资源的亲缘关系,结果比单标记测定更准确。分子标记类型的组合聚类分析显示出更高的遗传关系区分。根据 ISSR、SRAP、CBDP 和 CEAP 标记分析的组合,建议九个芒果种质可能是亲本或姐妹系。

更新日期:2022-04-21
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