Abstract

Investigations on the effects of newly constructed glucose-PEG2000-DSPE modified carbamazepine nano system on oxidative stress damage and cell apoptosis in epilepsy. The nano system was constructed by egg yolk lecithin, cholesterol, GLUPEG2000-DSPE, and carbamazepine as per the molar ratio of 95:20:5:6.35. The particle size, zeta potential, and release rate of carbamazepine was determined using a microscope and a microplate reader. The cells toxicity was detected for determine the optimal concentration of carbamazepine nano system. Cell uptake, cell apoptosis ratio and ROS level was determined by flow cytometry analysis. The in vivo studies were performed using male Wistar rats. H&E staining was employed for histological evaluation. Immunofluorescence was utilized for measure the expression level of GLUT1. ELISA assay was obtained for detecting the levels of SOD, GSH-Px and MDA. The results shown the average particle size was 108.57 ± 3.42 nm, and the mean zeta potential was −52.75 ± 1.48 mV. The modified carbamazepine liposomes exhibited higher release rate. Cell uptake indicated that carbamazepine nano system could be successfully internalized into cells. Flow cytometry analysis revealed the carbamazepine nano system dramatically decreased cell apoptosis rate and downregulated ROS level. Moreover, carbamazepine nano system improved histological status, increased GLUT1 expression and decreased oxidative stress in vivo. In conclusion, glucose-PEG2000-DSPE modified carbamazepine nano system ameliorated cell apoptosis and oxidative stress damage in epilepsy.

摘要

新型葡萄糖-PEG2000-DSPE修饰卡马西平纳米系统对癫痫氧化应激损伤及细胞凋亡的影响研究[J]. 该纳米体系由蛋黄卵磷脂、胆固醇、GLUPEG2000-DSPE和卡马西平按照95:20:5:6.35的摩尔比构建。使用显微镜和酶标仪测定卡马西平的粒径、zeta 电位和释放速率。检测细胞毒性以确定卡马西平纳米体系的最佳浓度。通过流式细胞术分析确定细胞摄取、细胞凋亡率和活性氧水平。使用雄性 Wistar 大鼠进行体内研究。H&E染色用于组织学评估。免疫荧光用于测量GLUT1的表达水平。获得ELISA法检测SOD、GSH-Px和MDA的水平。结果显示平均粒径为 108.57 ± 3.42 nm，平均 zeta 电位为 -52.75 ± 1.48 mV。修饰的卡马西平脂质体表现出更高的释放率。细胞摄取表明卡马西平纳米系统可以成功地内化到细胞中。流式细胞仪分析显示，卡马西平纳米系统显着降低了细胞凋亡率并下调了 ROS 水平。此外，卡马西平纳米系统改善了组织学状态，增加了体内 GLUT1 的表达并降低了氧化应激。总之，葡萄糖-PEG2000-DSPE修饰的卡马西平纳米系统改善了癫痫患者的细胞凋亡和氧化应激损伤。42 nm，平均 zeta 电位为 -52.75 ± 1.48 mV。修饰的卡马西平脂质体表现出更高的释放率。细胞摄取表明卡马西平纳米系统可以成功地内化到细胞中。流式细胞仪分析显示，卡马西平纳米系统显着降低了细胞凋亡率并下调了 ROS 水平。此外，卡马西平纳米系统改善了组织学状态，增加了体内 GLUT1 的表达并降低了氧化应激。总之，葡萄糖-PEG2000-DSPE修饰的卡马西平纳米系统改善了癫痫患者的细胞凋亡和氧化应激损伤。42 nm，平均 zeta 电位为 -52.75 ± 1.48 mV。修饰的卡马西平脂质体表现出更高的释放率。细胞摄取表明卡马西平纳米系统可以成功地内化到细胞中。流式细胞仪分析显示，卡马西平纳米系统显着降低了细胞凋亡率并下调了 ROS 水平。此外，卡马西平纳米系统改善了组织学状态，增加了体内 GLUT1 的表达并降低了氧化应激。总之，葡萄糖-PEG2000-DSPE修饰的卡马西平纳米系统改善了癫痫患者的细胞凋亡和氧化应激损伤。流式细胞仪分析显示，卡马西平纳米系统显着降低了细胞凋亡率并下调了 ROS 水平。此外，卡马西平纳米系统改善了组织学状态，增加了体内 GLUT1 的表达并降低了氧化应激。总之，葡萄糖-PEG2000-DSPE修饰的卡马西平纳米系统改善了癫痫患者的细胞凋亡和氧化应激损伤。流式细胞仪分析显示，卡马西平纳米系统显着降低了细胞凋亡率并下调了 ROS 水平。此外，卡马西平纳米系统改善了组织学状态，增加了体内 GLUT1 的表达并降低了氧化应激。总之，葡萄糖-PEG2000-DSPE修饰的卡马西平纳米系统改善了癫痫患者的细胞凋亡和氧化应激损伤。