The flatheaded root-borer Capnodis tenebrionis (Linné) (Coleoptera: Buprestidae) causes serious damage to stone fruit trees in Mediterranean countries. The use of entomopathogenic nematodes (EPNs) is an eco-friendly tool that can efficiently control C. tenebrionis. In this study, the efficacy of five EPN isolates native to Morocco was assessed against different developmental stages of C. tenebrionis in laboratory and greenhouse experiments. In Bioassay 1, the susceptibility of neonate larvae of C. tenebrionis to EPNs was evaluated according to temperature (25 and 32 °C) and soil moisture (5 and 10%). The greatest mortality rates were observed for Heterorhabditis bacteriophora HB-MOR7 (97.5%) and Steinernema feltiae SF-MOR9 (95%) with the 10% soil moisture-25 °C combination. S. feltiae SF-MOR9 and Heterorhabditis sp. HJo-MOR14 induced moderate to high mortality regardless of the temperature-soil moisture combination. In Bioassay 2, Heterorhabditis sp. HJo-MOR14 performed better against adults of C. tenebrionis than the other isolates. In addition, no difference in susceptibility between females and males was recorded for each EPN isolate. In pot experiment, more live larvae of C. tenebrionis were found in roots and collar of almond trees for HJo-MOR14 (0.93) than HB-MOR7 (0.47) and SF-MOR9 (0.43). Furthermore, treatments by SF-MOR9 and HB-MOR7 ensured low infestations of trees by larvae of C. tenebrionis than the treatment by HJo-MOR14 (36.7, 43.3 and 66.7% respectively). Our results show that Moroccan EPN isolates have potential as alternative methods for the control of C. tenebrionis.

扁头根螟Capnodis tenebrionis (Linné)（鞘翅目：Buprestidae）对地中海国家的核果树造成严重破坏。使用昆虫病原线虫 (EPN) 是一种环保工具，可以有效控制C. tenebrionis。在这项研究中，在实验室和温室实验中，针对不同发育阶段的C. tenebrionis评估了摩洛哥原产的五种 EPN 分离物的功效。在生物测定 1 中，根据温度（25 和 32 °C）和土壤湿度（5% 和 10%）评估C. tenebrionis新生幼虫对 EPNs 的敏感性。Heterorhabditis bacteriophora的死亡率最高HB-MOR7 (97.5%) 和Steinernema feeliae SF-MOR9 (95%) 与 10% 土壤湿度 - 25 °C 组合。S.毡氏SF-MOR9 和Heterorhabditis sp。无论温度-土壤水分组合如何，HJo-MOR14 都会诱导中度至高死亡率。在生物测定 2 中，Heterorhabditis sp。HJo-MOR14 对C. tenebrionis 成虫的表现优于其他分离株。此外，对于每个 EPN 分离物，没有记录到女性和男性之间的易感性差异。在盆栽试验中， C. tenebrionis的活幼虫较多HJo-MOR14 (0.93) 比 HB-MOR7 (0.47) 和 SF-MOR9 (0.43) 在杏仁树的根部和衣领中发现。此外，SF-MOR9 和 HB-MOR7 处理确保C. tenebrionis 幼虫对树木的侵扰低于 HJo-MOR14 处理（分别为 36.7、43.3 和 66.7%）。我们的结果表明，摩洛哥 EPN 分离株具有作为控制C. tenebrionis的替代方法的潜力。