This study was conducted to evaluate the effects of dietary heat-killed Lactobacillus plantarum and compound probiotics (GH35A and GH35B) on the growth performance, antioxidative capacity, intestinal morphology and microbiota of Litopenaeus vannamei. Shrimps (1.29 ± 0.01 g) were fed with five different diets: C (control, basal diet without probiotics), LP1 (basal diet supplemented with 0.05% heat-killed L. plantarum powder), LP2 (basal diet supplemented with 0.1% heat-killed L. plantarum powder), T1 (basal diet supplemented with 15% GH35A and 1% GH35B) and T2 (basal diet supplemented with 15% GH35A and 2% GH35B). After 28 days of the feeding trial, it was observed that the body weight gain (BWG), specific growth rate (SGR), condition factor (CF) and intestinal somatic index (ISI) were significantly higher in groups T1 and T2 compared to those observed in group C (p < 0.05). Compared with the control group, dietary supplementation of heat-killed L. plantarum and compound probiotics significantly influenced the activities of total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), peroxidase (POD), polyphenol oxidase (PPO), acid phosphatase (ACP) and alkaline phosphatase (AKP) in the hepatopancreas. The structure of intestinal villi in the groups LP2 and T2 was well-developed compared with the other treatments. The dominant phyla in L. vannamei were Proteobacteria and Bacteroidetes, and the abundances of Verrucomicrobia and Firmicutes were the lowest in C. Haloferula, which was the most abundant in LP2 and T1. Overall, dietary heat-killed L. plantarum and compound probiotics could positively influence the growth performance, antioxidative capacity, intestinal morphology and microbiota of L. vannamei.

中文翻译：

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日粮热灭活植物乳杆菌和复合益生菌对南美白对虾生长性能、抗氧化能力、肠道形态和微生物群的影响

本研究旨在评估日粮热灭活植物乳杆菌和复合益生菌（GH35A 和 GH35B）对南美白对虾生长性能、抗氧化能力、肠道形态和微生物群的影响。虾（1.29 ± 0.01 g）饲喂五种不同的日粮：C（对照，不含益生菌的基础日粮）、LP1（添加了 0.05% 热灭活植物乳杆菌粉的基础日粮）、LP2（添加了 0.1% 热量的基础日粮）-杀死植物乳杆菌粉）、T1（添加 15% GH35A 和 1% GH35B 的基础日粮）和 T2（添加 15% GH35A 和 2% GH35B 的基础日粮）。喂养试验28天后，观察到T1组和T2组的体重增加（BWG）、比生长率（SGR）、条件因子（CF）和肠道体细胞指数（ISI）明显高于T1组和T2组。在 C 组中观察到 ( p  < 0.05)。与对照组相比，膳食补充热灭活L . 植物复合益生菌显着影响总抗氧化能力（T-AOC）、总超氧化物歧化酶（T-SOD）、过氧化物酶（POD）、多酚氧化酶（PPO）、酸性磷酸酶（ACP）和碱性磷酸酶（AKP）的活性。肝胰腺。与其他处理相比，LP2和T2组的肠绒毛结构发育良好。L中的优势门。南美白杆菌属变形菌门和拟杆菌门，其中疣微菌和厚壁菌门的丰度在C. Haloferula中最低，在LP2和T1中丰度最高。总体而言，饮食热杀死L。植物复合益生菌可对L的生长性能、抗氧化能力、肠道形态和微生物群产生积极影响。南美白