Biosensors based on nucleic acid-structured electrochemiluminescence are rapidly developing for medical diagnostics. Here, we build an automated DNA molecular machine on Ti₃C₂/polyethyleneimine-Ru(dcbpy)₃²⁺@Au composite, which alters the situation that a DNA molecular machine requires laying down motion tracks. We use this DNA molecular machine to transduce the target concentration information to enhance the electrochemiluminescence signal based on DNA hybridization calculations. Complex bioanalytical processes are centralized in a single nucleic acid probe unit, thus eliminating the tedious steps of laying down motion tracks required by the traditional molecular machine. We found a detection limit of 0.68 pM and a range of 1 pM to 1 nM for the analysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specific DNA target. Recoveries range between 96.4 and 104.8% for the analysis of SARS-CoV-2 in human saliva.

基于核酸结构电化学发光的生物传感器正在迅速发展用于医学诊断。_{在这里，我们在 Ti 3} C ₂ /聚乙烯亚胺-Ru(dcbpy) ₃ ²⁺上构建了一个自动化的 DNA 分子机器@Au 复合材料，它改变了 DNA 分子机器需要铺设运动轨迹的情况。我们使用这种 DNA 分子机器来转换目标浓度信息，以增强基于 DNA 杂交计算的电化学发光信号。复杂的生物分析过程集中在单个核酸探针单元中，从而消除了传统分子机器所需的铺设运动轨迹的繁琐步骤。我们发现分析严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 特异性 DNA 靶标的检测限为 0.68 pM，范围为 1 pM 至 1 nM。人类唾液中 SARS-CoV-2 分析的回收率介于 96.4% 和 104.8% 之间。